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Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells

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dc.contributor.author Hammouda, Souha
dc.contributor.author Ghzaiel, Imen
dc.contributor.author Picón-Pagès, Pol
dc.contributor.author Meddeb, Wiem
dc.contributor.author Khamlaoui, Wided
dc.contributor.author Hammami, Sonia
dc.contributor.author Muñoz López, Francisco José, 1964-
dc.contributor.author Hammami, Mohamed
dc.contributor.author Zarrouk, Amira
dc.date.accessioned 2021-07-19T06:09:04Z
dc.date.available 2021-07-19T06:09:04Z
dc.date.issued 2021
dc.identifier.citation Hammouda S, Ghzaiel I, Picón-Pagès P, Meddeb W, Khamlaoui W, Hammami S, Muñoz FJ, Hammami M, Zarrouk A. Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells. Biomolecules. 2021;11(6):797. DOI: 10.3390/biom11060797
dc.identifier.issn 2218-273X
dc.identifier.uri http://hdl.handle.net/10230/48215
dc.description.abstract Oxysterols are assumed to be the driving force behind numerous neurodegenerative diseases. In this work, we aimed to study the ability of 7β-hydroxycholesterol (7β-OHC) to trigger oxidative stress and cell death in human neuroblastoma cells (SH-SY5Y) then the capacity of Nigella sativa and Milk thistle seed oils (NSO and MTSO, respectively) to oppose 7β-OHC-induced side effects. The impact of 7β-OHC, associated or not with NSO or MTSO, was studied on different criteria: cell viability; redox status, and apoptosis. Oxidative stress was assessed through the intracellular reactive oxygen species (ROS) production, levels of enzymatic and non-enzymatic antioxidants, lipid, and protein oxidation products. Our results indicate that 7β-OHC (40 µg/mL) exhibit pr-oxidative and pro-apoptotic activities shown by a decrease of the antioxidant enzymatic activities and an increase of ROS production, lipid, and protein oxidation end products as well as nitrotyrosine formation and caspase 3 activation. However, under the pre-treatment with NSO, and especially with MTSO (100 µg/mL), a marked attenuation of oxidative damages was observed. Our study suggests harmful effects of 7β-OHC consisting of pro-oxidative, anti-proliferative, and pro-apoptotic activities that may contribute to neurodegeneration. NSO and especially MTSO showed potential cytoprotection against the cytotoxicity of 7β-OHC.
dc.description.sponsorship This study was supported by the Ministry of Higher Education and Scientific Research (MHESR), Tunisia. This work was also supported by the Spanish Ministry of Science and Innovation and FEDER Funds through grants SAF2017-83372-R (FJM) and through the “María de Maeztu” Program for Units of Excellence in R&D from Spain (award CEX2018-000792-M).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof Biomolecules. 2021;11(6):797
dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3390/biom11060797
dc.subject.keyword 7β-hydroxycholesterol
dc.subject.keyword Antioxidants
dc.subject.keyword Antioxidants enzymes
dc.subject.keyword Cellular oxidative stress
dc.subject.keyword Neuroblastoma cells
dc.subject.keyword Neurodegenertion
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2017-83372-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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