Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells

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• dc.contributor.author Hammouda, Souha
• dc.contributor.author Ghzaiel, Imen
• dc.contributor.author Picón-Pagès, Pol
• dc.contributor.author Meddeb, Wiem
• dc.contributor.author Khamlaoui, Wided
• dc.contributor.author Hammami, Sonia
• dc.contributor.author Muñoz López, Francisco José, 1964-
• dc.contributor.author Hammami, Mohamed
• dc.contributor.author Zarrouk, Amira
• dc.date.accessioned 2021-07-19T06:09:04Z
• dc.date.available 2021-07-19T06:09:04Z
• dc.date.issued 2021
• dc.description.abstract Oxysterols are assumed to be the driving force behind numerous neurodegenerative diseases. In this work, we aimed to study the ability of 7β-hydroxycholesterol (7β-OHC) to trigger oxidative stress and cell death in human neuroblastoma cells (SH-SY5Y) then the capacity of Nigella sativa and Milk thistle seed oils (NSO and MTSO, respectively) to oppose 7β-OHC-induced side effects. The impact of 7β-OHC, associated or not with NSO or MTSO, was studied on different criteria: cell viability; redox status, and apoptosis. Oxidative stress was assessed through the intracellular reactive oxygen species (ROS) production, levels of enzymatic and non-enzymatic antioxidants, lipid, and protein oxidation products. Our results indicate that 7β-OHC (40 µg/mL) exhibit pr-oxidative and pro-apoptotic activities shown by a decrease of the antioxidant enzymatic activities and an increase of ROS production, lipid, and protein oxidation end products as well as nitrotyrosine formation and caspase 3 activation. However, under the pre-treatment with NSO, and especially with MTSO (100 µg/mL), a marked attenuation of oxidative damages was observed. Our study suggests harmful effects of 7β-OHC consisting of pro-oxidative, anti-proliferative, and pro-apoptotic activities that may contribute to neurodegeneration. NSO and especially MTSO showed potential cytoprotection against the cytotoxicity of 7β-OHC.
• dc.description.sponsorship This study was supported by the Ministry of Higher Education and Scientific Research (MHESR), Tunisia. This work was also supported by the Spanish Ministry of Science and Innovation and FEDER Funds through grants SAF2017-83372-R (FJM) and through the “María de Maeztu” Program for Units of Excellence in R&D from Spain (award CEX2018-000792-M).
• dc.format.mimetype application/pdf
• dc.identifier.citation Hammouda S, Ghzaiel I, Picón-Pagès P, Meddeb W, Khamlaoui W, Hammami S, Muñoz FJ, Hammami M, Zarrouk A. Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells. Biomolecules. 2021;11(6):797. DOI: 10.3390/biom11060797
• dc.identifier.doi http://dx.doi.org/10.3390/biom11060797
• dc.identifier.issn 2218-273X
• dc.identifier.uri http://hdl.handle.net/10230/48215
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Biomolecules. 2021;11(6):797
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2017-83372-R
• dc.rights © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword 7β-hydroxycholesterol
• dc.subject.keyword Antioxidants
• dc.subject.keyword Antioxidants enzymes
• dc.subject.keyword Cellular oxidative stress
• dc.subject.keyword Neuroblastoma cells
• dc.subject.keyword Neurodegenertion
• dc.title Nigella and milk thistle seed oils: potential cytoprotective effects against 7β-hydroxycholesterol-induced toxicity on SH-SY5Y cells
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion