dc.contributor.author |
López Trujillo, Emilio |
dc.contributor.author |
Gonzàlez Farré, Mònica |
dc.contributor.author |
Pujol, Ramón M. |
dc.contributor.author |
Bellosillo Paricio, Beatriz |
dc.contributor.author |
Fisa, Roser |
dc.contributor.author |
Riera Carro, Cristina |
dc.contributor.author |
Alcover, Magdalena |
dc.contributor.author |
Barranco Sanz, Luis Carlos |
dc.contributor.author |
Martin-Ezquerra, Gemma |
dc.date.accessioned |
2021-06-17T06:17:52Z |
dc.date.available |
2021-06-17T06:17:52Z |
dc.date.issued |
2021 |
dc.identifier.citation |
Lopez-Trujillo E, Gonzàlez-Farré M, Pujol RM, Bellosillo B, Fisa R, Riera C, Alcover M, Barranco C, Martin-Ezquerra G. Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis. Histol Histopathol. 2021;36(5):567-76. DOI: 10.14670/HH-18-324 |
dc.identifier.issn |
0213-3911 |
dc.identifier.uri |
http://hdl.handle.net/10230/47913 |
dc.description.abstract |
Background: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness. Objectives: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis. Materials and methods: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results: From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, anti-CD1a and anti-Leishmania, was 94% [CI 95%: (79,8%-99,3%)] ; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. Conclusions: Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin - eosin and Giemsa staining. |
dc.format.mimetype |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Histology and Histopathology |
dc.relation.ispartof |
Histol Histopathol. 2021;36(5):567-76 |
dc.rights |
© The Author(s) 2021. Open Access. This article is licensed under a Creative Commons CC-BY International License. |
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
dc.title |
Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis |
dc.type |
info:eu-repo/semantics/article |
dc.identifier.doi |
http://dx.doi.org/10.14670/HH-18-324 |
dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
dc.type.version |
info:eu-repo/semantics/publishedVersion |