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Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis

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dc.contributor.author López Trujillo, Emilio
dc.contributor.author Gonzàlez Farré, Mònica
dc.contributor.author Pujol, Ramón M.
dc.contributor.author Bellosillo Paricio, Beatriz
dc.contributor.author Fisa, Roser
dc.contributor.author Riera Carro, Cristina
dc.contributor.author Alcover, Magdalena
dc.contributor.author Barranco Sanz, Luis Carlos
dc.contributor.author Martin-Ezquerra, Gemma
dc.date.accessioned 2021-06-17T06:17:52Z
dc.date.available 2021-06-17T06:17:52Z
dc.date.issued 2021
dc.identifier.citation Lopez-Trujillo E, Gonzàlez-Farré M, Pujol RM, Bellosillo B, Fisa R, Riera C, Alcover M, Barranco C, Martin-Ezquerra G. Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis. Histol Histopathol. 2021;36(5):567-76. DOI: 10.14670/HH-18-324
dc.identifier.issn 0213-3911
dc.identifier.uri http://hdl.handle.net/10230/47913
dc.description.abstract Background: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness. Objectives: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis. Materials and methods: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results: From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, anti-CD1a and anti-Leishmania, was 94% [CI 95%: (79,8%-99,3%)] ; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. Conclusions: Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin - eosin and Giemsa staining.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Histology and Histopathology
dc.relation.ispartof Histol Histopathol. 2021;36(5):567-76
dc.rights © The Author(s) 2021. Open Access. This article is licensed under a Creative Commons CC-BY International License.
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.14670/HH-18-324
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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