Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis

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• dc.contributor.author López Trujillo, Emilio
• dc.contributor.author Gonzàlez Farré, Mònica
• dc.contributor.author Pujol Vallverdú, Ramon Maria
• dc.contributor.author Bellosillo Paricio, Beatriz
• dc.contributor.author Fisa, Roser
• dc.contributor.author Riera Carro, Cristina
• dc.contributor.author Alcover, Magdalena
• dc.contributor.author Barranco Sanz, Luis Carlos
• dc.contributor.author Martin-Ezquerra, Gemma
• dc.date.accessioned 2021-06-17T06:17:52Z
• dc.date.available 2021-06-17T06:17:52Z
• dc.date.issued 2021
• dc.description.abstract Background: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness. Objectives: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis. Materials and methods: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results: From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, anti-CD1a and anti-Leishmania, was 94% [CI 95%: (79,8%-99,3%)] ; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. Conclusions: Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin - eosin and Giemsa staining.
• dc.format.mimetype application/pdf
• dc.identifier.citation Lopez-Trujillo E, Gonzàlez-Farré M, Pujol RM, Bellosillo B, Fisa R, Riera C, et al. Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis. Histol Histopathol. 2021 May;36(5):567-76. DOI: 10.14670/HH-18-324
• dc.identifier.doi http://dx.doi.org/10.14670/HH-18-324
• dc.identifier.issn 0213-3911
• dc.identifier.uri http://hdl.handle.net/10230/47913
• dc.language.iso eng
• dc.publisher Histology and Histopathology
• dc.relation.ispartof Histol Histopathol. 2021;36(5):567-76
• dc.rights © The Author(s) 2021. Open Access. This article is licensed under a Creative Commons CC-BY International License (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by/4.0/
• dc.title Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion