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Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts

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dc.contributor.author Ugarte Corbalán, Laura de, 1988-
dc.contributor.author Balcells, Susana
dc.contributor.author Güerri Fernández, Roberto
dc.contributor.author Grinberg, Daniel
dc.contributor.author Díez Pérez, Adolfo
dc.contributor.author Nogués Solan, Francesc Xavier
dc.contributor.author Garcia Giralt, Natàlia
dc.date.accessioned 2021-02-24T07:42:26Z
dc.date.available 2021-02-24T07:42:26Z
dc.date.issued 2020
dc.identifier.citation Ugarte L de, Balcells S, Guerri-Fernandez R, Grinberg D, Diez-Perez A, Nogues X, Garcia-Giralt N. Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts. Appl. Sci. 2020; 10(8):2852. DOI: 10.3390/app10082852
dc.identifier.issn 2076-3417
dc.identifier.uri http://hdl.handle.net/10230/46579
dc.description.abstract The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.
dc.description.sponsorship This research was supported by the CIBER on Frailty and Healthy Ageing (CIBERFES; grant number: CB16/10/00245), the CIBERER (grant number: U720), FEDER funds, and grants from the Science and Innovation Ministry (ISCIII; grant numbers: PI16/01860 and PI13/00116; SAF2016-75948-R). L.D.-U. was granted with a PFIS predoctoral fellowship from the ISCIII.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher MDPI
dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3390/app10082852
dc.subject.keyword Osteosarcoma
dc.subject.keyword MG-63
dc.subject.keyword U2OS
dc.subject.keyword Primary osteoblasts
dc.subject.keyword miR-320a
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-75948-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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