Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts

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• dc.contributor.author Ugarte Corbalán, Laura de, 1988-
• dc.contributor.author Balcells, Susana
• dc.contributor.author Güerri Fernández, Roberto
• dc.contributor.author Grinberg, Daniel
• dc.contributor.author Díez Pérez, Adolfo
• dc.contributor.author Nogués Solan, Francesc Xavier
• dc.contributor.author Garcia Giralt, Natàlia
• dc.date.accessioned 2021-02-24T07:42:26Z
• dc.date.available 2021-02-24T07:42:26Z
• dc.date.issued 2020
• dc.description.abstract The miR-320a regulates a number of genes involved in various physiological processes. In particular, it has been reported as a tumor suppressor in several types of human cancers and involved in osteoporotic fracture and osteoblast function. Hence, the role of miR-320a has been evaluated in tumor cells and in primary cells in a separated context, but its effect has never been explored in a comparative manner. The present study aims to evaluate the cellular effects of miR-320a on human osteosarcoma cell lines (MG-63 and U2OS) compared to that on primary human osteoblasts (hOBs). miR-320a was either overexpressed or inhibited in all cell lines, and cell proliferation and viability were analyzed. Additionally, the effects of miR-320a on matrix mineralization, alkaline phosphatase activity, and oxidative stress were also evaluated in order to assess osteoblast functionality. In osteosarcoma cells, miR-320a overexpression reduced cell viability and proliferation, while in hOB cell viability was not affected and proliferation even was increased. The overexpression of miR-320a in both osteosarcoma cells and hOBs reduced the mineralization capacity. Finally, an increased oxidative stress was detected in all cells after miR-320a overexpression mainly in osteosarcoma. In conclusion, the overexpression of miR-320a increased stress oxidation levels, which could be involved in the reduced osteoblast performance, even though the cell viability was only affected in osteosarcoma cells.
• dc.description.sponsorship This research was supported by the CIBER on Frailty and Healthy Ageing (CIBERFES; grant number: CB16/10/00245), the CIBERER (grant number: U720), FEDER funds, and grants from the Science and Innovation Ministry (ISCIII; grant numbers: PI16/01860 and PI13/00116; SAF2016-75948-R). L.D.-U. was granted with a PFIS predoctoral fellowship from the ISCIII.
• dc.format.mimetype application/pdf
• dc.identifier.citation Ugarte L de, Balcells S, Guerri-Fernandez R, Grinberg D, Diez-Perez A, Nogues X, Garcia-Giralt N. Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts. Appl. Sci. 2020; 10(8):2852. DOI: 10.3390/app10082852
• dc.identifier.doi http://dx.doi.org/10.3390/app10082852
• dc.identifier.issn 2076-3417
• dc.identifier.uri http://hdl.handle.net/10230/46579
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-75948-R
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Osteosarcoma
• dc.subject.keyword MG-63
• dc.subject.keyword U2OS
• dc.subject.keyword Primary osteoblasts
• dc.subject.keyword miR-320a
• dc.title Effect of the tumor suppressor miR-320a on viability and functionality of human osteosarcoma cell Llnes compared to primary osteoblasts
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion