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Pulsed radiofrequency for chronic pain: In vitro evidence of an electroporation mediated calcium uptake

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dc.contributor.author Mercadal, Borja
dc.contributor.author Vicente García, Rubén, 1978-
dc.contributor.author Ivorra Cano, Antoni, 1974-
dc.date.accessioned 2020-08-25T08:25:52Z
dc.date.issued 2020
dc.identifier.citation Mercadal B, Vicente R, Ivorra A. Pulsed radiofrequency for chronic pain: In vitro evidence of an electroporation mediated calcium uptake. Bioelectrochemistry. 2020;136:107624. DOI: 10.1016/j.bioelechem.2020.107624
dc.identifier.issn 1567-5394
dc.identifier.uri http://hdl.handle.net/10230/45235
dc.description.abstract Pulsed radiofrequency (PRF) treatments for chronic pain consist in the delivery of a train of sinusoidal electric bursts to the targeted nerve. Despite numerous clinical evidence of its efficiency, the mechanism of action of PRF remains unclear. Since most of the reported biological effects of PRF can be initiated by a calcium influx into the neurons, we hypothesized that PRF may induce a mild electroporation effect causing a calcium uptake. To test this hypothesis, HEK-293 cells were exposed to PRF bursts and cytosolic calcium and Yo-Pro-1 uptake were monitored. After a single burst, calcium peaks were observed for electric fields above 480 V/cm while the uptake of Yo-pro-1 was insignificant. After a train of 120 bursts, the electric fields required to induce a calcium and Yo-pro-1 uptake decreased to 330 V/cm and 880 V/cm respectively. Calcium peaks were not detected when cells were treated in calcium free media. The temperature increase during the treatments was lower than 5 °C in all cases. Finally, the cell response for different burst frequencies and extracellular media conductivities correlated with the induced transmembrane voltage calculated with a numerical model. Our results support the hypothesis of an electroporation mediated calcium influx.
dc.description.sponsorship This work was supported by the Ministry of Economy and Competitiveness of Spain through the grants TEC2014-52383-C3-2-R and SAF2014-52228-R. Antoni Ivorra gratefully acknowledges the financial support by ICREA under the ICREA Academia programme.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Bioelectrochemistry. 2020;136:107624.
dc.rights © Elsevier http://dx.doi.org/10.1016/j.bioelechem.2020.107624
dc.title Pulsed radiofrequency for chronic pain: In vitro evidence of an electroporation mediated calcium uptake
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.bioelechem.2020.107624
dc.subject.keyword Sine waves
dc.subject.keyword Neuropathic pain
dc.subject.keyword Neuromodulation
dc.subject.keyword Cell membrane
dc.subject.keyword Electric fields
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-52228-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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