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Dynamics of cell death after conventional IRE and H-FIRE treatments

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dc.contributor.author Mercadal, Borja
dc.contributor.author Beitel White, Natalie
dc.contributor.author Aycock, Kenneth N.
dc.contributor.author Castellví Fernández, Quim
dc.contributor.author Davalos, Rafael Vidal
dc.contributor.author Ivorra Cano, Antoni, 1974-
dc.date.accessioned 2020-03-12T15:41:24Z
dc.date.available 2020-03-12T15:41:24Z
dc.date.issued 2020
dc.identifier.citation Mercadal B, Beitel-White N, Aycock KN, Castellví Q, Davalos RV, Ivorra A. Dynamics of cell death after conventional IRE and H-FIRE treatments. Ann Biomed Eng. 2020. DOI: 10.1007/s10439-020-02462-8
dc.identifier.issn 0090-6964
dc.identifier.uri http://hdl.handle.net/10230/43888
dc.description.abstract High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.
dc.description.sponsorship This work was supported by the Ministry of Economy and Competitiveness of Spain (Grant No. TEC2014-52383-C3-2-R), the Pancreatic Cancer Action Network (Grant No. 16-65-IANN) and Cures Within Reach (Grant No. PUJFSANY). Antoni Ivorra gratefully acknowledges financial support by ICREA under the ICREA Academia programme.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Annals of Biomedical Engineering. 2020
dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Dynamics of cell death after conventional IRE and H-FIRE treatments
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1007/s10439-020-02462-8
dc.subject.keyword Irreversible electroporation
dc.subject.keyword High-frequency irreversible electroporation
dc.subject.keyword Membrane permeability
dc.subject.keyword Caspase 3/7
dc.subject.keyword Bipolar pulses
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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