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Membrane attack complex and factor H in humans with acute kidney injury

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dc.contributor.author Rodriguez García, Eva
dc.contributor.author Gimeno Beltran, Javier
dc.contributor.author Arias Cabrales, Carlos Enrique
dc.contributor.author Barrios Barrera, Clara
dc.contributor.author Redondo Pachón, María Dolores
dc.contributor.author Soler, María José
dc.contributor.author Crespo Barrio, Marta
dc.contributor.author Sierra Ochoa, Adriana
dc.contributor.author Riera Oliva, Marta
dc.contributor.author Pascual Santos, Julio
dc.date.accessioned 2019-06-06T07:15:56Z
dc.date.available 2019-06-06T07:15:56Z
dc.date.issued 2018
dc.identifier.citation Rodríguez E, Gimeno J, Arias-Cabrales C, Barrios C, Redondo-Pachón D, Soler MJ. et al. Membrane attack complex and factor H in humans with acute kidney injury. Kidney Blood Press Res. 2018;43(5):1655-1665. DOI: 10.1159/000494680
dc.identifier.issn 1420-4096
dc.identifier.uri http://hdl.handle.net/10230/41712
dc.description.abstract BACKGROUND AND AIMS: Complement system seems to play an important role in the pathogenesis of Acute Kidney Injury (AKI). The aim of this study was to investigate the role of complement system in the pathogenesis of human AKI. For this purpose, we studied Membrane Attack Complex (MAC) and factor H in plasma and kidney tissue in AKI. METHODS: Plasmatic concentrations of MAC and Factor H were studied in patients with hospital-acquired AKI and their respective controls. MAC and Factor H expression and localization within the kidney were studied by immunohistochemistry in kidney tissue samples from autopsies. Demographical, past medical, and laboratory data in patients on admission and 3 years after discharge were recorded. RESULTS: Plasmatic MAC concentrations were significantly higher in AKI-patients (5848±3604 vs 3703±1483 mAU/mL, p< 0.01), mainly in the severe cases, as measured by the need of renal replacement therapy, non-recovery of renal function, RIFLE classification and CKD development. MAC deposition was observed in tubular epithelial cell basal membranes, showing a larger number of tubules with MAC deposition, larger perimeter of affected tubules and greater intensity of MAC immunostaining in AKI patients. Factor H concentrations were higher in AKI patients (0.86±0.05 vs 0.60±0.04 mg/mL, p=0.007), showing a strong positive association with plasmatic MAC (r=0.7, p< 0.01)). Factor H immunostaining showed a tubular cytoplasmic pattern, with significant variations in the staining intensity, associated with the severity of histologic damage. CONCLUSION: Our data confirm that complement system is involved in human AKI, through the lytic action of MAC in tubular epithelial cells. These results suggest that complement system activation in AKI could be related with CKD development.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Karger (S. Karger AG)
dc.rights This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) https://creativecommons.org/licenses/by-nc-nd/4.0/. Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title Membrane attack complex and factor H in humans with acute kidney injury
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1159/0004946
dc.subject.keyword AKI
dc.subject.keyword Complement System
dc.subject.keyword Factor H
dc.subject.keyword Membrane Attack Complex (MAC)
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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