Membrane attack complex and factor H in humans with acute kidney injury

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• dc.contributor.author Rodriguez García, Eva
• dc.contributor.author Gimeno Beltran, Javier
• dc.contributor.author Arias Cabrales, Carlos Enrique
• dc.contributor.author Barrios Barrera, Clara
• dc.contributor.author Redondo Pachón, María Dolores
• dc.contributor.author Soler, María José
• dc.contributor.author Crespo Barrio, Marta
• dc.contributor.author Sierra Ochoa, Adriana
• dc.contributor.author Riera Oliva, Marta
• dc.contributor.author Pascual Santos, Julio
• dc.date.accessioned 2019-06-06T07:15:56Z
• dc.date.available 2019-06-06T07:15:56Z
• dc.date.issued 2018
• dc.description.abstract BACKGROUND AND AIMS: Complement system seems to play an important role in the pathogenesis of Acute Kidney Injury (AKI). The aim of this study was to investigate the role of complement system in the pathogenesis of human AKI. For this purpose, we studied Membrane Attack Complex (MAC) and factor H in plasma and kidney tissue in AKI. METHODS: Plasmatic concentrations of MAC and Factor H were studied in patients with hospital-acquired AKI and their respective controls. MAC and Factor H expression and localization within the kidney were studied by immunohistochemistry in kidney tissue samples from autopsies. Demographical, past medical, and laboratory data in patients on admission and 3 years after discharge were recorded. RESULTS: Plasmatic MAC concentrations were significantly higher in AKI-patients (5848±3604 vs 3703±1483 mAU/mL, p< 0.01), mainly in the severe cases, as measured by the need of renal replacement therapy, non-recovery of renal function, RIFLE classification and CKD development. MAC deposition was observed in tubular epithelial cell basal membranes, showing a larger number of tubules with MAC deposition, larger perimeter of affected tubules and greater intensity of MAC immunostaining in AKI patients. Factor H concentrations were higher in AKI patients (0.86±0.05 vs 0.60±0.04 mg/mL, p=0.007), showing a strong positive association with plasmatic MAC (r=0.7, p< 0.01)). Factor H immunostaining showed a tubular cytoplasmic pattern, with significant variations in the staining intensity, associated with the severity of histologic damage. CONCLUSION: Our data confirm that complement system is involved in human AKI, through the lytic action of MAC in tubular epithelial cells. These results suggest that complement system activation in AKI could be related with CKD development.
• dc.format.mimetype application/pdf
• dc.identifier.citation Rodríguez E, Gimeno J, Arias-Cabrales C, Barrios C, Redondo-Pachón D, Soler MJ. et al. Membrane attack complex and factor H in humans with acute kidney injury. Kidney Blood Press Res. 2018;43(5):1655-1665. DOI: 10.1159/000494680
• dc.identifier.doi http://dx.doi.org/10.1159/0004946
• dc.identifier.issn 1420-4096
• dc.identifier.uri http://hdl.handle.net/10230/41712
• dc.language.iso eng
• dc.publisher Karger (S. Karger AG)
• dc.rights This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) https://creativecommons.org/licenses/by-nc-nd/4.0/. Usage and distribution for commercial purposes as well as any distribution of modified material requires written permission.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword AKI
• dc.subject.keyword Complement System
• dc.subject.keyword Factor H
• dc.subject.keyword Membrane Attack Complex (MAC)
• dc.title Membrane attack complex and factor H in humans with acute kidney injury
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion