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ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells

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dc.contributor.author Riso, Vincenzo
dc.contributor.author Cammisa, Marco
dc.contributor.author Kukreja, Harpreet
dc.contributor.author Anvar, Zahra
dc.contributor.author Verde, Gaetano
dc.contributor.author Sparago, Angela
dc.contributor.author Acurzio, Basilia
dc.contributor.author Lad, Shraddha
dc.contributor.author Lonardo, Enza
dc.contributor.author Sankar, Aditya
dc.contributor.author Helin, Kristian
dc.contributor.author Feil, Robert
dc.contributor.author Fico, Annalisa
dc.contributor.author Angelini, Claudia
dc.contributor.author Grimaldi, Giovanna
dc.contributor.author Riccio, Andrea
dc.date.accessioned 2018-02-02T08:34:41Z
dc.date.available 2018-02-02T08:34:41Z
dc.date.issued 2016
dc.identifier.citation Riso V, Cammisa M, Kukreja H, Anvar Z, Verde G, Sparago A. et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res. 2016 Sep 30;44(17):8165-78. DOI: 10.1093/nar/gkw505
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10230/33798
dc.description.abstract ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inbred and hybrid mouse ESC lines carrying different gene knockouts. By using an allele-specific RNA-seq approach, we demonstrate that ZFP57 loss results in derepression of the imprinted allele of multiple genes in the imprinted clusters. We also find marked epigenetic differences between ICRBS and non-ICRBS suggesting that different cis-acting regulatory functions are repressed by ZFP57 at these two classes of target loci. Overall, these data demonstrate that ZFP57 is pivotal to maintain the allele-specific epigenetic modifications of ICRs that in turn are necessary for maintaining the imprinted expression over long distances. At non-ICRBS, ZFP57 inactivation results in acquisition of epigenetic features that are characteristic of poised enhancers, suggesting that another function of ZFP57 in early embryogenesis is to repress cis-acting regulatory elements whose activity is not yet required.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof Nucleic Acids Research. 2016 Sep 30;44(17):8165-78
dc.rights Copyright © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
dc.subject.other Epigènesi
dc.subject.other Animals -- Aspectes genètics
dc.subject.other Cèl·lules -- Proliferació
dc.subject.other Histones
dc.title ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkw505
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/290123
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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