ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells

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• dc.contributor.author Riso, Vincenzoca
• dc.contributor.author Cammisa, Marcoca
• dc.contributor.author Kukreja, Harpreetca
• dc.contributor.author Anvar, Zahraca
• dc.contributor.author Verde, Gaetanoca
• dc.contributor.author Sparago, Angelaca
• dc.contributor.author Acurzio, Basiliaca
• dc.contributor.author Lad, Shraddhaca
• dc.contributor.author Lonardo, Enzaca
• dc.contributor.author Sankar, Adityaca
• dc.contributor.author Helin, Kristianca
• dc.contributor.author Feil, Robertca
• dc.contributor.author Fico, Annalisaca
• dc.contributor.author Angelini, Claudiaca
• dc.contributor.author Grimaldi, Giovannaca
• dc.contributor.author Riccio, Andreaca
• dc.date.accessioned 2018-02-02T08:34:41Z
• dc.date.available 2018-02-02T08:34:41Z
• dc.date.issued 2016
• dc.description.abstract ZFP57 is necessary for maintaining repressive epigenetic modifications at Imprinting control regions (ICRs). In mouse embryonic stem cells (ESCs), ZFP57 binds ICRs (ICRBS) and many other loci (non-ICRBS). To address the role of ZFP57 on all its target sites, we performed high-throughput and multi-locus analyses of inbred and hybrid mouse ESC lines carrying different gene knockouts. By using an allele-specific RNA-seq approach, we demonstrate that ZFP57 loss results in derepression of the imprinted allele of multiple genes in the imprinted clusters. We also find marked epigenetic differences between ICRBS and non-ICRBS suggesting that different cis-acting regulatory functions are repressed by ZFP57 at these two classes of target loci. Overall, these data demonstrate that ZFP57 is pivotal to maintain the allele-specific epigenetic modifications of ICRs that in turn are necessary for maintaining the imprinted expression over long distances. At non-ICRBS, ZFP57 inactivation results in acquisition of epigenetic features that are characteristic of poised enhancers, suggesting that another function of ZFP57 in early embryogenesis is to repress cis-acting regulatory elements whose activity is not yet required.
• dc.format.mimetype application/pdf
• dc.identifier.citation Riso V, Cammisa M, Kukreja H, Anvar Z, Verde G, Sparago A. et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res. 2016 Sep 30;44(17):8165-78. DOI: 10.1093/nar/gkw505
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkw505
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/33798
• dc.language.iso eng
• dc.publisher Oxford University Pressca
• dc.relation.ispartof Nucleic Acids Research. 2016 Sep 30;44(17):8165-78
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/290123
• dc.rights Copyright © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Epigènesi
• dc.subject.other Animals -- Aspectes genètics
• dc.subject.other Cèl·lules -- Proliferació
• dc.subject.other Histones
• dc.title ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cellsca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion