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Long-term effectiveness of irreversible electroporation in a murine model of colorectal liver metastasis

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dc.contributor.author Ivorra Cano, Antoni, 1974-
dc.contributor.author Sánchez Velázquez, Patricia, 1985-
dc.contributor.author Castellví Fernández, Quim
dc.contributor.author Villanueva, Alberto
dc.contributor.author Iglesias Coma, Mar
dc.contributor.author Quesada Diez, Rita
dc.contributor.author Pañella Vilamú, Clara, 1987-
dc.contributor.author Cáceres Aguilar, Mario
dc.contributor.author Dorcaratto, Dimitri
dc.contributor.author Andaluz, Anna
dc.contributor.author Moll, Xavier
dc.contributor.author Burdío, José M.
dc.contributor.author Grande Posa, Luís
dc.contributor.author Burdío Pinilla, Fernando
dc.date.accessioned 2017-05-16T08:18:53Z
dc.date.available 2017-05-16T08:18:53Z
dc.date.issued 2017
dc.identifier.citation Sánchez-Velázquez P, Cstellví Q, Villanueva A, Iglesias M, Quesada R, Pañella C, Cáceres M, Dorcaratto D, Andaluz A, Moll X, Burdío JM, Grande L, Ivorra A, Burdío F. Long-term effectiveness of irreversible electroporation in a murine model of colorectal liver metastasis. Sci Rep. 2017;7:44821. DOI: 10.1038/srep44821
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/32129
dc.description.abstract Irreversible electroporation (IRE) has recently gained in popularity as an ablative technique, however little is known about its oncological long-term outcomes. To determine the long-time survival of animals treated with a high dose of IRE and which histological changes it induces in tumoral tissue, IRE ablation was performed in forty-six athymic-nude mice with KM12C tumors implanted in the liver by applying electric current with different voltages (2000 V/cm, 1000 V/cm). The tumors were allowed to continue to grow until the animals reached the end-point criteria. Histology was harvested and the extent of tumor necrosis was semi-quantitatively assessed. IRE treatment with the 2000 V/cm protocol significantly prolonged median mouse survival from 74.3 ± 6.9 days in the sham group to 112.5 ± 15.2 days in the 2000 V/cm group. No differences were observed between the mean survival of the 1000 V/cm and the sham group (83.2 ± 16.4 days, p = 0.62). Histology revealed 63.05% ± 23.12 of tumor necrosis in animals of the 2000 V/cm group as compared to 17.50% ± 2.50 in the 1000 V/cm group and 25.6% ± 22.1 in the Sham group (p = 0.001). IRE prolonged the survival of animals treated with the highest electric field (2000 V/cm). The animals in this group showed significantly higher rate of tumoral necrosis.
dc.description.sponsorship This research was supported by the spanish government under grants TEC2011-27133-c02 and TEC2010-17285, and by the spanish “plan estatal de investigación, desarrollo e innovación orientada a los retos de la sociedad” under grant TEC2014–52383-c3-r (TEC2014–52383-c3-2-r and TEC2014–52383-c3-3-r).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.relation.ispartof Scientific Reports. 2017;7:44821.
dc.rights This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ © The Author(s) 2017
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Long-term effectiveness of irreversible electroporation in a murine model of colorectal liver metastasis
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/srep44821
dc.subject.keyword Cancer models
dc.subject.keyword Surgical oncology
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/TEC2011-27133-C02
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/TEC2010-17285
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014–52383-C3-R
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014–52383-C3-2-R
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014–52383-C3-3-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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