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Irreversible electroporation of the liver: is there a safe limit to the ablation volume?

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dc.contributor.author Sánchez-Velázquez, Joan
dc.contributor.author Castellví Fernández, Quim
dc.contributor.author Villanueva, Alberto
dc.contributor.author Quesada Diez, Rita
dc.contributor.author Pañella Vilamú, Clara, 1987-
dc.contributor.author Cáceres Aguilar, Mario
dc.contributor.author Dorcaratto, Dimitri
dc.contributor.author Andaluz, Anna
dc.contributor.author Moll, Xavier
dc.contributor.author Trujillo Guillén, Macarena
dc.contributor.author Burdío, José M.
dc.contributor.author Berjano, Enrique J.
dc.contributor.author Grande Posa, Luís
dc.contributor.author Ivorra Cano, Antoni, 1974-
dc.contributor.author Burdío Pinilla, Fernando
dc.date.accessioned 2016-04-05T15:39:52Z
dc.date.available 2016-04-05T15:39:52Z
dc.date.issued 2016-04-01
dc.identifier.citation Sánchez-Velázquez P, Castellví Q, Villanueva A, Quesada R, Pañella C, Cáceres M, Dorcaratto C, Andaluz A, Moll X, Trujillo M, Burdío JM, Berjano E, Grande L, Ivorra A, Burdío F. Irreversible electroporation of the liver: is there a safe limit to the ablation volume? Scientific reports. 2016;6:23781. DOI: 10.1038/srep23781
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/26048
dc.description.abstract Irreversible electroporation is a fast-growing liver ablation technique. Although safety has been well documented in small ablations, our aim is to assess its safety and feasibility when a large portion of liver is ablated. Eighty-seven mice were subjected to high voltage pulses directly delivered across parallel plate electrodes comprising around 40% of mouse liver. One group consisted in 55 athymic-nude, in which a tumor from the KM12C cell line was grown and the other thirty-two C57-Bl6 non-tumoral mice. Both groups were subsequently divided into subsets according to the delivered field /nstrength (1000V/cm, 2000V/cm) and whether or not they received anti-hyperkalemia therapy. Early mortality (less than 24hours post-IRE) in the 2000V/cm group was observed and revealed considerably higher mean potassium levels. In contrast, the animals subjected to a 2000V/cm field treated with the anti-hyperkalemia therapy had higher survival rates (OR=0.1, 95%CI=0.02–0.32, p<0.001). Early mortality also depended on the electric field magnitude of the IRE protocol, as mice given 1000V/cm survived longer than those given 2000V/cm (OR=4.7, 95%CI=1.8–11.8, p=0.001). Our findings suggest that ionic disturbances, mainly due to potassium alterations, should be warned and envisioned when large volume ablations are performed by IRE.
dc.description.sponsorship This research was supported by the Spanish “Plan Estatal de Investigación, Desarrollo e Innovación Orientada/na los Retos de la Sociedad” under Grant TEC2014–52383-C3 (TEC2014-52383-C3-1-R, TEC2014-52383-C3-/n2-R, and TEC2014-52383-C3-3-R), and by the Spanish “Plan Nacional de I + D + i del Ministerio de Ciencia e/nInnovación” under Grants TEC2011-27133-C02 and TEC2010-17285.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.relation.ispartof Scientific reports. 2016;6:23781.
dc.rights This work is licensed under a Creative Commons Attribution 4.0 International License. The images other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Irreversible electroporation of the liver: is there a safe limit to the ablation volume?
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/srep23781
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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