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The HOG pathway dictates the short-term translational response after hyperosmotic shock

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dc.contributor.author Warringer, Jonas
dc.contributor.author Hult, Malin
dc.contributor.author Regot Rodríguez de Mier, Sergi
dc.contributor.author Posas Garriga, Francesc
dc.contributor.author Sunnerhagen, Per
dc.date.accessioned 2016-01-18T16:42:26Z
dc.date.available 2016-01-18T16:42:26Z
dc.date.issued 2010
dc.identifier.citation Warringer J, Hult M, Regot S, Posas F, Sunnerhagen P. The HOG pathway dictates the short-term translational response after hyperosmotic shock. Molecular biology of the cell. 2010; 21(17): 3080-3092. DOI 10.1091/mbc.E10-01-0006
dc.identifier.issn 1059-1524
dc.identifier.uri http://hdl.handle.net/10230/25595
dc.description.abstract Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins including hexose transporters were enriched. Similarly, numerous mRNAs encoding cytoplasmic ribosomal proteins run counter to the overall trend of down-regulation and are instead translationally mobilized late in the response. Surprisingly, certain transcriptionally induced mRNAs were excluded from ribosomal association after shock. Importantly, we verify, using constructs with intact 5' and 3' untranslated regions, that the observed changes in polysomal mRNA are reflected in protein levels, including cases with only translational up-regulation. Interestingly, the translational regulation of the most highly osmostress-regulated mRNAs was more strongly dependent on the stress-activated protein kinases Hog1 and Rck2 than the transcriptional regulation. Our results show the importance of translational control for fine tuning of the adaptive responses.
dc.description.sponsorship This work was financially supported by the European Commission (QUASI; LSHG-CT2003-530203 and UNICELLSYS; LSHG-CT2007-201142) to P.S and F.P, grants from MICINN and Consolider from the Spanish government, EURYI scheme award (www.esf.org/euryi), ICREA Acadèmia (Generalitat de Catalunya) and Fundación Marcelino Botín (FMB) to F.P, and by grants from the Swedish Research Council (2007-5460) and the Swedish Cancer Fund (09-0772) to P. S.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher American Society for Cell Biology
dc.relation.ispartof Molecular biology of the cell. 2010; 21(17): 3080-3092
dc.rights © 2012 Warringer et al. This article is under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License
dc.rights.uri http://creativecommons.org/licenses/by-ncsa/3.0
dc.subject.other Saccharomyces cerevisiae
dc.subject.other Membranes cel·lulars
dc.title The HOG pathway dictates the short-term translational response after hyperosmotic shock
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1091/mbc.E10-01-0006
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/530203
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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