The HOG pathway dictates the short-term translational response after hyperosmotic shock
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- dc.contributor.author Warringer, Jonasca
- dc.contributor.author Hult, Malinca
- dc.contributor.author Regot Rodríguez de Mier, Sergica
- dc.contributor.author Posas Garriga, Francescca
- dc.contributor.author Sunnerhagen, Perca
- dc.date.accessioned 2016-01-18T16:42:26Z
- dc.date.available 2016-01-18T16:42:26Z
- dc.date.issued 2010
- dc.description.abstract Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins including hexose transporters were enriched. Similarly, numerous mRNAs encoding cytoplasmic ribosomal proteins run counter to the overall trend of down-regulation and are instead translationally mobilized late in the response. Surprisingly, certain transcriptionally induced mRNAs were excluded from ribosomal association after shock. Importantly, we verify, using constructs with intact 5' and 3' untranslated regions, that the observed changes in polysomal mRNA are reflected in protein levels, including cases with only translational up-regulation. Interestingly, the translational regulation of the most highly osmostress-regulated mRNAs was more strongly dependent on the stress-activated protein kinases Hog1 and Rck2 than the transcriptional regulation. Our results show the importance of translational control for fine tuning of the adaptive responses.ca
- dc.description.sponsorship This work was financially supported by the European Commission (QUASI; LSHG-CT2003-530203 and UNICELLSYS; LSHG-CT2007-201142) to P.S and F.P, grants from MICINN and Consolider from the Spanish government, EURYI scheme award (www.esf.org/euryi), ICREA Acadèmia (Generalitat de Catalunya) and Fundación Marcelino Botín (FMB) to F.P, and by grants from the Swedish Research Council (2007-5460) and the Swedish Cancer Fund (09-0772) to P. S.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Warringer J, Hult M, Regot S, Posas F, Sunnerhagen P. The HOG pathway dictates the short-term translational response after hyperosmotic shock. Molecular biology of the cell. 2010; 21(17): 3080-3092. DOI 10.1091/mbc.E10-01-0006ca
- dc.identifier.doi http://dx.doi.org/10.1091/mbc.E10-01-0006
- dc.identifier.issn 1059-1524
- dc.identifier.uri http://hdl.handle.net/10230/25595
- dc.language.iso engca
- dc.publisher American Society for Cell Biologyca
- dc.relation.ispartof Molecular biology of the cell. 2010; 21(17): 3080-3092
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/530203
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
- dc.rights © 2012 Warringer et al. This article is under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons Licenseca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri http://creativecommons.org/licenses/by-ncsa/3.0ca
- dc.subject.other Saccharomyces cerevisiaeca
- dc.subject.other Membranes cel·lularsca
- dc.title The HOG pathway dictates the short-term translational response after hyperosmotic shockca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca