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A p120-catenin-CK1epsilon complex regulates Wnt signaling

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dc.contributor.author Casagolda, David
dc.contributor.author Valle Pérez, Beatriz del
dc.contributor.author Valls, Gabriela
dc.contributor.author Lugilde, Ero
dc.contributor.author Vinyoles, Meritxell
dc.contributor.author Casado Vela, Juan
dc.contributor.author Solanas, Guiomar
dc.contributor.author Batlle Gómez, Eduard
dc.contributor.author Reynolds, Albert B.
dc.contributor.author Casal, José Ignacio
dc.contributor.author García de Herreros, Antonio
dc.contributor.author Duñach, Mireia
dc.date.accessioned 2016-01-15T19:41:45Z
dc.date.available 2016-01-15T19:41:45Z
dc.date.issued 2010
dc.identifier.citation Casagolda D, Del Valle-Pérez B, Valls G, Lugilde E, Vinyoles M, Casado-Vela J. A p120-catenin-CK1epsilon complex regulates Wnt signaling. Journal of cell science. 2010; 123(Pt 15): 2621-2631. DOI 10.1242/jcs.067512
dc.identifier.issn 1214-021X
dc.identifier.uri http://hdl.handle.net/10230/25587
dc.description.abstract p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction mediated by E-cadherin, showing an unexpected physical link between adherens junctions and a Wnt receptor. Depletion of p120-catenin abolishes CK1ε binding to LRP5/6 and prevents CK1ε activation upon Wnt3a stimulation. Elimination of p120-catenin also inhibits early responses to Wnt, such as LRP5/6 and Dvl-2 phosphorylation and axin recruitment to the signalosome, as well as later effects, such as β-catenin stabilization. Moreover, since CK1ε is also required for E-cadherin phosphorylation, a modification that decreases the affinity for β-catenin, p120-catenin depletion prevents the increase in β-catenin transcriptional activity even in the absence of β-catenin degradation. Therefore, these results demonstrate a novel and crucial function of p120-catenin in Wnt signaling and unveil additional points of regulation by this factor of β-catenin transcriptional activity different of β-catenin stability.
dc.description.sponsorship This study was supported by grants awarded by the Ministerio de Ciencia e Innovación: BFU2006-03203 and BFU2009-07578 to M.D. and SAF2006-00339 to A.G.H. Partial support from the Instituto Carlos III (RD06/0020/0040) and the Generalitat de Catalunya (2009SGR867 and 2009SGR585) is also appreciated. D.C. and G.V. were supported by predoctoral fellowships from the Ministerio de Educación and E.L., from the UAB.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Company of Biologists
dc.relation.ispartof Journal of cell science. 2010; 123(Pt 15): 2621-2631
dc.rights © Company of Biologists http://jcs.biologists.org/content/joces/123/15/2621.full.pdf DOI 10.1242/jcs.067512
dc.subject.other Proteïnes quinases -- Metabolisme
dc.subject.other Cadherines
dc.title A p120-catenin-CK1epsilon complex regulates Wnt signaling
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1242/jcs.067512
dc.subject.keyword CK1
dc.subject.keyword E-cadherin
dc.subject.keyword Wnt
dc.subject.keyword p120-catenin
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2006-03203
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/SAF2006-00339
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-07578
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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