dc.contributor.author |
Casagolda, David |
dc.contributor.author |
Valle Pérez, Beatriz del |
dc.contributor.author |
Valls, Gabriela |
dc.contributor.author |
Lugilde, Ero |
dc.contributor.author |
Vinyoles, Meritxell |
dc.contributor.author |
Casado Vela, Juan |
dc.contributor.author |
Solanas, Guiomar |
dc.contributor.author |
Batlle Gómez, Eduard |
dc.contributor.author |
Reynolds, Albert B. |
dc.contributor.author |
Casal, José Ignacio |
dc.contributor.author |
García de Herreros, Antonio |
dc.contributor.author |
Duñach, Mireia |
dc.date.accessioned |
2016-01-15T19:41:45Z |
dc.date.available |
2016-01-15T19:41:45Z |
dc.date.issued |
2010 |
dc.identifier.citation |
Casagolda D, Del Valle-Pérez B, Valls G, Lugilde E, Vinyoles M, Casado-Vela J. A p120-catenin-CK1epsilon complex regulates Wnt signaling. Journal of cell science. 2010; 123(Pt 15): 2621-2631. DOI 10.1242/jcs.067512 |
dc.identifier.issn |
1214-021X |
dc.identifier.uri |
http://hdl.handle.net/10230/25587 |
dc.description.abstract |
p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction mediated by E-cadherin, showing an unexpected physical link between adherens junctions and a Wnt receptor. Depletion of p120-catenin abolishes CK1ε binding to LRP5/6 and prevents CK1ε activation upon Wnt3a stimulation. Elimination of p120-catenin also inhibits early responses to Wnt, such as LRP5/6 and Dvl-2 phosphorylation and axin recruitment to the signalosome, as well as later effects, such as β-catenin stabilization. Moreover, since CK1ε is also required for E-cadherin phosphorylation, a modification that decreases the affinity for β-catenin, p120-catenin depletion prevents the increase in β-catenin transcriptional activity even in the absence of β-catenin degradation. Therefore, these results demonstrate a novel and crucial function of p120-catenin in Wnt signaling and unveil additional points of regulation by this factor of β-catenin transcriptional activity different of β-catenin stability. |
dc.description.sponsorship |
This study was supported by grants awarded by the Ministerio de Ciencia e Innovación: BFU2006-03203 and BFU2009-07578 to M.D. and SAF2006-00339 to A.G.H. Partial support from the Instituto Carlos III (RD06/0020/0040) and the Generalitat de Catalunya (2009SGR867 and 2009SGR585) is also appreciated. D.C. and G.V. were supported by predoctoral fellowships from the Ministerio de Educación and E.L., from the UAB. |
dc.format.mimetype |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Company of Biologists |
dc.relation.ispartof |
Journal of cell science. 2010; 123(Pt 15): 2621-2631 |
dc.rights |
© Company of Biologists http://jcs.biologists.org/content/joces/123/15/2621.full.pdf DOI 10.1242/jcs.067512 |
dc.subject.other |
Proteïnes quinases -- Metabolisme |
dc.subject.other |
Cadherines |
dc.title |
A p120-catenin-CK1epsilon complex regulates Wnt signaling |
dc.type |
info:eu-repo/semantics/article |
dc.identifier.doi |
http://dx.doi.org/10.1242/jcs.067512 |
dc.subject.keyword |
CK1 |
dc.subject.keyword |
E-cadherin |
dc.subject.keyword |
Wnt |
dc.subject.keyword |
p120-catenin |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/2PN/BFU2006-03203 |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/2PN/SAF2006-00339 |
dc.relation.projectID |
info:eu-repo/grantAgreement/ES/3PN/BFU2009-07578 |
dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
dc.type.version |
info:eu-repo/semantics/publishedVersion |