A p120-catenin-CK1epsilon complex regulates Wnt signaling
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- dc.contributor.author Casagolda, Davidca
- dc.contributor.author Valle Pérez, Beatriz delca
- dc.contributor.author Valls, Gabrielaca
- dc.contributor.author Lugilde, Eroca
- dc.contributor.author Vinyoles, Meritxellca
- dc.contributor.author Casado Vela, Juanca
- dc.contributor.author Solanas, Guiomarca
- dc.contributor.author Batlle Gómez, Eduardca
- dc.contributor.author Reynolds, Albert B.ca
- dc.contributor.author Casal, José Ignacioca
- dc.contributor.author García de Herreros, Antonioca
- dc.contributor.author Duñach, Mireiaca
- dc.date.accessioned 2016-01-15T19:41:45Z
- dc.date.available 2016-01-15T19:41:45Z
- dc.date.issued 2010
- dc.description.abstract p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction mediated by E-cadherin, showing an unexpected physical link between adherens junctions and a Wnt receptor. Depletion of p120-catenin abolishes CK1ε binding to LRP5/6 and prevents CK1ε activation upon Wnt3a stimulation. Elimination of p120-catenin also inhibits early responses to Wnt, such as LRP5/6 and Dvl-2 phosphorylation and axin recruitment to the signalosome, as well as later effects, such as β-catenin stabilization. Moreover, since CK1ε is also required for E-cadherin phosphorylation, a modification that decreases the affinity for β-catenin, p120-catenin depletion prevents the increase in β-catenin transcriptional activity even in the absence of β-catenin degradation. Therefore, these results demonstrate a novel and crucial function of p120-catenin in Wnt signaling and unveil additional points of regulation by this factor of β-catenin transcriptional activity different of β-catenin stability.ca
- dc.description.sponsorship This study was supported by grants awarded by the Ministerio de Ciencia e Innovación: BFU2006-03203 and BFU2009-07578 to M.D. and SAF2006-00339 to A.G.H. Partial support from the Instituto Carlos III (RD06/0020/0040) and the Generalitat de Catalunya (2009SGR867 and 2009SGR585) is also appreciated. D.C. and G.V. were supported by predoctoral fellowships from the Ministerio de Educación and E.L., from the UAB.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Casagolda D, Del Valle-Pérez B, Valls G, Lugilde E, Vinyoles M, Casado-Vela J. A p120-catenin-CK1epsilon complex regulates Wnt signaling. Journal of cell science. 2010; 123(Pt 15): 2621-2631. DOI 10.1242/jcs.067512ca
- dc.identifier.doi http://dx.doi.org/10.1242/jcs.067512
- dc.identifier.issn 1214-021X
- dc.identifier.uri http://hdl.handle.net/10230/25587
- dc.language.iso engca
- dc.publisher Company of Biologistsca
- dc.relation.ispartof Journal of cell science. 2010; 123(Pt 15): 2621-2631
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/BFU2006-03203
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/SAF2006-00339
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-07578
- dc.rights © Company of Biologists http://jcs.biologists.org/content/joces/123/15/2621.full.pdf DOI 10.1242/jcs.067512ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.keyword CK1
- dc.subject.keyword E-cadherin
- dc.subject.keyword Wnt
- dc.subject.keyword p120-catenin
- dc.subject.other Proteïnes quinases -- Metabolismeca
- dc.subject.other Cadherinesca
- dc.title A p120-catenin-CK1epsilon complex regulates Wnt signalingca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca