Temperature affects all processes, including development, and yet, it remains unknown how and/or if underlying mechanical and biochemical events are coordinated to slow down development robustly at lower temperatures. Here, using a variety of methods from molecular biology to 5D live imaging, I uncover the vulnerabilities of the zebrafish epithelial during gastrulation at sub-optimal temperatures (20° C). The enveloping layer (EVL) in the cold displays large multi-nucleated cells stemming from cytokinesis failures. Differences in tissue tension during late epiboly also led to rupturing of the EVL in certain embryos. Furthermore, mesendoderm patterning reveals convergence-extension defects under varying temperatures, suggesting that different aspects of gastrulation are affected in parallel. Proteome profiling across five different temperatures also reveals processes affected under temperature fluctuations. This PhD project is one of the first studies focusing on the effect of temperature on gastrulation and exposes mechanisms behind developmental failures under sub-optimal temperatures.
(Universitat Pompeu Fabra, 2024-07-12T14:24:36Z) Lim, Jia Le; Trivedi, Vikas; Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida
