Leukemic involvement is a common feature in waldenström macroglobulinemia at diagnosis

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• dc.contributor.author Montesdeoca, Sara
• dc.contributor.author García Gisbert, Nieves, 1994-
• dc.contributor.author Calvo, Xavier
• dc.contributor.author Arenillas Rocha, Leonor
• dc.contributor.author Román, David
• dc.contributor.author Fernández Rodríguez, M. Concepción
• dc.contributor.author Navarro, Rosa
• dc.contributor.author Costan, Beatriz
• dc.contributor.author Vela Ortíz, María del Carmen
• dc.contributor.author Camacho Díaz, Laura
• dc.contributor.author Abella Monreal, Eugenia
• dc.contributor.author Colomo Saperas, Luis Alberto
• dc.contributor.author Salido Galeote, Marta
• dc.contributor.author Puiggros Metje, Anna Maria
• dc.contributor.author Florensa Brichs, Lourdes
• dc.contributor.author Espinet Solà, Blanca
• dc.contributor.author Bellosillo Paricio, Beatriz
• dc.contributor.author Ferrer del Álamo, Anna
• dc.date.accessioned 2024-04-30T06:12:40Z
• dc.date.available 2024-04-30T06:12:40Z
• dc.date.issued 2023
• dc.description.abstract Waldenström Macroglobulinemia (WM) is a lymphoplasmacytic lymphoma with bone marrow (BM) involvement and IgM monoclonal gammopathy. To date, no studies have focused specifically on peripheral blood (PB) involvement. In this study, 100 patients diagnosed with WM according to the World Health Organization (WHO) criteria were included based on the demonstration of MYD88mut in BM and the availability of PB multiparametric flow cytometry (MFC) analysis. Leukemic involvement by MFC was detected in 50/100 patients. A low percentage of mature small lymphocytes in PB smears was observed in only 15 cases. MYD88mut by AS-qPCR was detected in PB in 65/100 cases. In cases with leukemic expression by MFC, MYD88mut was detected in all cases, and IGH was rearranged in 44/49 cases. In 21/50 patients without PB involvement by MFC, molecular data were consistent with circulating disease (MYD88mut by AS-qPCR 3/50, IGH rearranged 6/50, both 12/50). Therefore, PB involvement by standard techniques was detected in 71/100 patients. MYD88mut was detected in PB by dPCR in 9/29 triple negative cases. Overall, 80% of the patients presented PB involvement by any technique. Our findings support the role of PB MFC in the evaluation of patients with IgM monoclonal gammopathy and provide reliable information on correlation with molecular features. The development of a feasible MFC assay may stand as an objective tool in the classification of mature B cell neoplasms presenting with IgM monoclonal gammopathy.
• dc.format.mimetype application/pdf
• dc.identifier.citation Montesdeoca S, García-Gisbert N, Calvo X, Arenillas L, Román D, Fernández-Rodríguez C, et al. Leukemic involvement is a common feature in waldenström macroglobulinemia at diagnosis. Cancers (Basel). 2023 Aug 17;15(16):4152. DOI: 10.3390/cancers15164152
• dc.identifier.doi http://dx.doi.org/10.3390/cancers15164152
• dc.identifier.issn 2072-6694
• dc.identifier.uri http://hdl.handle.net/10230/59949
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Cancers (Basel). 2023 Aug 17;15(16):4152
• dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword MFC
• dc.subject.keyword MYD88 L265P
• dc.subject.keyword Waldenström Macroglobulinemia (WM)
• dc.subject.keyword dPCR
• dc.subject.keyword Flow cytometry
• dc.subject.keyword Peripheral blood involvement
• dc.title Leukemic involvement is a common feature in waldenström macroglobulinemia at diagnosis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion