Species-specific segmentation clock periods are due to differential biochemical reaction speeds

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• dc.contributor.author Matsuda, Mitsuhiro
• dc.contributor.author Hayashi, Hanako
• dc.contributor.author García Ojalvo, Jordi
• dc.contributor.author Yoshioka-Kobayashi, Kumiko
• dc.contributor.author Kageyama, Ryoichiro
• dc.contributor.author Yamanaka, Yoshihiro
• dc.contributor.author Ikeya, Makoto
• dc.contributor.author Toguchida, Junya
• dc.contributor.author Alev, Cantas
• dc.contributor.author Ebisuya, Miki
• dc.date.accessioned 2022-01-26T07:34:13Z
• dc.date.available 2022-01-26T07:34:13Z
• dc.date.issued 2020
• dc.description.abstract Although mechanisms of embryonic development are similar between mice and humans, the time scale is generally slower in humans. To investigate these interspecies differences in development, we recapitulate murine and human segmentation clocks that display 2- to 3-hour and 5- to 6-hour oscillation periods, respectively. Our interspecies genome-swapping analyses indicate that the period difference is not due to sequence differences in the HES7 locus, the core gene of the segmentation clock. Instead, we demonstrate that multiple biochemical reactions of HES7, including the degradation and expression delays, are slower in human cells than they are in mouse cells. With the measured biochemical parameters, our mathematical model accounts for the two- to threefold period difference between the species. We propose that cell-autonomous differences in biochemical reaction speeds underlie temporal differences in development between species.
• dc.format.mimetype application/pdf
• dc.identifier.citation Matsuda M, Hayashi H, Garcia-Ojalvo J, Yoshioka-Kobayashi K, Kageyama R, Yamanaka Y, Ikeya M, Toguchida J, Alev C, Ebisuya M. Species-specific segmentation clock periods are due to differential biochemical reaction speeds. Science. 2020;369(6510):1450-5. DOI: 10.1126/science.aba7668
• dc.identifier.doi http://dx.doi.org/10.1126/science.aba7668
• dc.identifier.issn 0036-8075
• dc.identifier.uri http://hdl.handle.net/10230/52326
• dc.language.iso eng
• dc.publisher American Association for the Advancement of Science (AAAS)
• dc.relation.ispartof Science. 2020;369(6510):1450-5
• dc.rights This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Matsuda M, Hayashi H, Garcia-Ojalvo J, Yoshioka-Kobayashi K, Kageyama R, Yamanaka Y, Ikeya M, Toguchida J, Alev C, Ebisuya M. Species-specific segmentation clock periods are due to differential biochemical reaction speeds. Science. 2020;369(6510):1450-5. DOI: 10.1126/science.aba7668
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.title Species-specific segmentation clock periods are due to differential biochemical reaction speeds
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion