Endothelial cell activation on 3D-matrices derived from PDGF-BB-stimulated fibroblasts is mediated by Snail1

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• dc.contributor.author Herrera, Alberto
• dc.contributor.author Herrera, Mercedes
• dc.contributor.author Guerra-Perez, Natalia
• dc.contributor.author Galindo-Pumariño, Cristina
• dc.contributor.author Larriba, María Jesús
• dc.contributor.author García-Barberán, Vanesa
• dc.contributor.author Gil, Beatriz
• dc.contributor.author Giménez-Moyano, Sara
• dc.contributor.author Ferreiro-Monteagudo, Reyes
• dc.contributor.author Veguillas, Pilar
• dc.contributor.author Candia, Antonio
• dc.contributor.author Peña, Raúl
• dc.contributor.author Pinto, Jesús
• dc.contributor.author García-Bermejo, Mª Laura
• dc.contributor.author Muñoz, Alberto
• dc.contributor.author García de Herreros, Antonio
• dc.contributor.author Bonilla, Félix
• dc.contributor.author Carrato, Alfredo
• dc.contributor.author Peña, Cristina
• dc.date.accessioned 2019-04-03T07:34:57Z
• dc.date.available 2019-04-03T07:34:57Z
• dc.date.issued 2018
• dc.description.abstract Carcinomas, such as colon cancer, initiate their invasion by rescuing the innate plasticity of both epithelial cells and stromal cells. Although Snail is a transcriptional factor involved in the Epithelial-Mesenchymal Transition, in recent years, many studies have also identified the major role of Snail in the activation of Cancer-Associated Fibroblast (CAF) cells and the remodeling of the extracellular matrix. In CAFs, Platelet-derived growth factor (PDGF) receptor signaling is a major functional determinant. High expression of both SNAI1 and PDGF receptors is associated with poor prognosis in cancer patients, but the mechanism(s) that underlie these connections are not understood. In this study, we demonstrate that PDGF-activated fibroblasts stimulate extracellular matrix (ECM) fiber remodeling and deposition. Furthermore, we describe how SNAI1, through the FAK pathway, is a necessary factor for ECM fiber organization. The parallel-oriented fibers are used by endothelial cells as "tracks", facilitating their activation and the creation of tubular structures mimicking in vivo capillary formation. Accordingly, Snail1 expression in fibroblasts was required for the co-adjuvant effect of these cells on matrix remodeling and neoangiogenesis when co-xenografted in nude mice. Finally, in tumor samples from colorectal cancer patients a direct association between stromal SNAI1 expression and the endothelial marker CD34 was observed. In summary, our results advance the understanding of PDGF/SNAI1-activated CAFs in matrix remodeling and angiogenesis stimulation.
• dc.description.sponsorship This research is supported by PI12/02037, PI12/01635, PI15/02101, PI17/01847, RD12/0036/0041 and RD12/0036/0021, and PIE15/00008 from the Instituto de Salud Carlos III- by Fondo Europeo de Desarrollo Regional (FEDER); by “CIBER de Cáncer”, CB16/12/00273, CB16/1200301, and CB16/12/00446, from the Instituto de Salud Carlos III-FEDER; by the Fundación Científica AECC (a multifaceted approach to target pancreatic cancer); by SAF2010-20750 and SAF2016-76461-R from the Ministerio de Economía y Competitividad of Spain-FEDER; by S2010/BMD-2344 from the Comunidad de Madrid; and by the Fundación Banco Santander. A.G.de.H.’ laboratory is supported by RD12/0036/0005 (from the Instituto de Salud Carlos III-FEDER) and SAF2013-48849-C2-1-R and SAF2016-76377-R (from the Ministerio de Economía y Competitividad of Spain-FEDER). C.P. is a recipient of a Miguel Servet Contract from the Instituto de Salud Carlos III. M. Eaude helped with the English text. We thank lab members for help and advice throughout this research.
• dc.format.mimetype application/pdf
• dc.identifier.citation Herrera A, Herrera M, Guerra-Perez N, Galindo-Pumariño C, Larriba MJ, García-Barberán V, Gil B, Giménez-Moyano S, Ferreiro-Monteagudo R, Veguillas P, Candia A, Peña R, Pinto J, García-Bermejo ML, Muñoz A, García de Herreros A, Bonilla F, Carrato A, Peña C. Endothelial cell activation on 3D-matrices derived from PDGF-BB-stimulated fibroblasts is mediated by Snail1. Oncogenesis. 2018; 7(9):76. DOI 10.1038/s41389-018-0085-z
• dc.identifier.doi http://dx.doi.org/10.1038/s41389-018-0085-z
• dc.identifier.issn 2157-9024
• dc.identifier.uri http://hdl.handle.net/10230/37032
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Oncogenesis. 2018; 7(9):76
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2010-20750
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-76461-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2013-48849-C2-1-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-76377-R
• dc.rights © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cancer microenvironment
• dc.subject.keyword Colorectal cancer
• dc.subject.keyword Tumour angiogenesis
• dc.title Endothelial cell activation on 3D-matrices derived from PDGF-BB-stimulated fibroblasts is mediated by Snail1
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion