Real-time protein and cell binding measurements on hydroxyapatite coatings.
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- dc.contributor.author Vilardell, A. M.ca
- dc.contributor.author Cinca, Núriaca
- dc.contributor.author Jokinen, A.ca
- dc.contributor.author Garcia Giralt, Natàliaca
- dc.contributor.author Dosta, Sergica
- dc.contributor.author Cano, Irene G.ca
- dc.contributor.author Guilemany, José Mariaca
- dc.date.accessioned 2017-01-17T12:33:27Z
- dc.date.available 2017-01-17T12:33:27Z
- dc.date.issued 2016
- dc.description.abstract Although a lot of in vitro and in vivo assays have been performed during the last few decades years for hydroxyapatite bioactive coatings, there is a lack of exploitation of real-time in vitro interaction measurements. In the present work, real-time interactions for a plasma sprayed hydroxyapatite coating were measured by a Multi-Parametric Surface Plasmon Resonance (MP-SPR), and the results were compared with standard traditional cell viability in vitro assays. MP-SPR is proven to be suitable not only for measurement of molecule-molecule interactions but also molecule-material interaction measurements and cell interaction. Although SPR is extensively utilized in interaction studies, recent research of protein or cell adsorption on hydroxyapatite coatings for prostheses applications was not found. The as-sprayed hydroxyapatite coating resulted in 62.4% of crystalline phase and an average thickness of 24 ± 6 μm. The MP-SPR was used to measure lysozyme protein and human mesenchymal stem cells interaction to the hydroxyapatite coating. A comparison between the standard gold sensor and Hydroxyapatite (HA)-plasma coated sensor denoted a clearly favourable cell attachment on HA coated sensor as a significantly higher signal of cell binding was detected. Moreover, traditional cell viability and proliferation tests showed increased activity with culture time indicating that cells were proliferating on HA coating. Cells show homogeneous distribution and proliferation along the HA surface between one and seven days with no significant mortality. Cells were flattened and spread on rough surfaces from the first day, with increasing cytoplasmatic extensions during the culture time.ca
- dc.description.sponsorship The authors wish to thank the Generalitat de Catalunya for the project 2014 SGR 1558 and the University of Barcelona for the award of a scholarship that has allowed the development of this research. The authors thank the Adult Stem Cell Group, BioMediTech (University of Tampere, Tampere, Finland) for collaboration and providing the cells for the MP-SPR measurement. The authors wish to thank Department of Automation Science and Engineering (Tampere University of Technology, Tampere, Finland) and Niko Granqvist from BioNavis for their help related to MP-SPR measurements.
- dc.format.mimetype application/pdfca
- dc.identifier.citation Vilardell AM, Cinca N, Jokinen A, Garcia-Giralt N, Dosta S, Cano IG. et al. Real-time protein and cell binding measurements on hydroxyapatite coatings. J Funct Biomater. 2016 Aug 27;7(3). pii: E23. doi: 10.3390/jfb7030023ca
- dc.identifier.doi http://dx.doi.org/10.3390/jfb7030023
- dc.identifier.issn 2079-4983
- dc.identifier.uri http://hdl.handle.net/10230/27914
- dc.language.iso engca
- dc.publisher MDPI AGca
- dc.relation.ispartof Journal of Functional Biomaterials. 2016 Aug 27;7(3):E23
- dc.rights © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/ca
- dc.subject.other Assaigs clínicsca
- dc.subject.other hidroxiapatitaca
- dc.title Real-time protein and cell binding measurements on hydroxyapatite coatings.ca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca