Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Barreiro Portela, Esther
- dc.contributor.author Salazar Degracia, Anna
- dc.contributor.author Sancho Muñoz, Antonio
- dc.contributor.author Gea Guiral, Joaquim
- dc.date.accessioned 2019-05-31T07:28:53Z
- dc.date.issued 2019
- dc.description.abstract mpaired muscle strength and mass (sarcopenia) are common in patients with respiratory cachexia, namely chronic obstructive pulmonary disease (COPD) and in lung cancer (LC)-cachexia. Misfolded/unfolded proteins in endoplasmic reticulum (ER) induce the compensatory unfolded protein response (UPR). Expression of ER stress and UPR markers may be differentially upregulated in vastus lateralis (VL) of patients with respiratory sarcopenia associated with either a chronic condition (COPD) or subacute (LC)-cachexia. In VL specimens from 40 COPD patients (n = 21, sarcopenic, fat-free mass index [FFMI] 16 kg/m2 and n = 19, nonsarcopenic, FFMI 18 kg/m2 ), 13 patients with LC-cachexia (FFMI 17 kg/m2 ), and 19 healthy controls (FFMI 19 kg/m 2 ), expression markers of ER stress, UPR (protein kinase-like ER kinase [PERK], activating transcription factor [ATF] 6, and inositol-requiring enzyme [IRE] 1-α), oxidative stress, autophagy, proteolysis, and apoptosis (reverse transcription polymerase chain reaction and immunoblotting), and fiber atrophy (histology) were assessed. Atrophy and muscle wasting and weakness were seen in both groups of sarcopenic patients. Compared to healthy controls, in muscles of LC-cachexia patients, expression of ER stress markers and UPR (three arms) was significantly upregulated, while in sarcopenic COPD, expression of a few ER stress markers and IRE1-α arm was upregulated. ER stress and an exaggerated UPR were observed in the VL muscle of patients with respiratory sarcopenia. The three branches of UPR were similarly upregulated in muscles of cancer cachectic patients, whereas in sarcopenic COPD patients, only IRE1 was upregulated. The differential profile of muscle UPR in chronic and subacute respiratory conditions offers a niche for the design of specific novel therapeutic approaches.
- dc.format.mimetype application/pdf
- dc.identifier.citation Barreiro E, Salazar-Degracia A, Sancho-Muñoz A, Gea J. Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases. J Cell Physiol. 2019 Jul;234(7):11315-29. DOI: 10.1002/jcp.27789
- dc.identifier.doi http://dx.doi.org/10.1002/jcp.27789
- dc.identifier.issn 0021-9541
- dc.identifier.uri http://hdl.handle.net/10230/41675
- dc.language.iso eng
- dc.publisher Wiley
- dc.relation.ispartof Journal of Cellular Physiology. 2019 Jul;234(7):11315-29
- dc.rights This is the peer reviewed version of the following article: Barreiro E, Salazar-Degracia A, Sancho-Muñoz A, Gea J. Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases. J Cell Physiol. 2019 Jul;234(7):11315-11329, which has been published in final form at http://dx.doi.org/10.1002/jcp.27789. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword COPD
- dc.subject.keyword Chronic and subacute respiratory sarcopenic conditions
- dc.subject.keyword Endoplasmic reticulum stress
- dc.subject.keyword Lower limb muscles
- dc.subject.keyword Lung cancer
- dc.subject.keyword Unfolded protein response
- dc.subject.other Pulmons -- Malalties obstructives
- dc.title Endoplasmic reticulum stress and unfolded protein response profile in quadriceps of sarcopenic patients with respiratory diseases
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion