The exocyst in context

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• dc.contributor.author Meek, Sasha
• dc.contributor.author Hernández, Altair C.
• dc.contributor.author Oliva Miguel, Baldomero
• dc.contributor.author Gallego, Oriol
• dc.date.accessioned 2025-03-26T07:33:17Z
• dc.date.available 2025-03-26T07:33:17Z
• dc.date.issued 2024
• dc.description.abstract The exocyst is a hetero-octameric complex involved in the exocytosis arm of cellular trafficking. Specifically, it tethers secretory vesicles to the plasma membrane, but it is also a main convergence point for many players of exocytosis: regulatory proteins, motor proteins, lipids and Soluble N-ethylmaleimide-sensitive factor Attachment Protein Receptor (SNARE) proteins are all connected physically by the exocyst. Despite extensive knowledge about its structure and interactions, the exocyst remains an enigma precisely because of its increasingly broad and flexible role across the exocytosis process. To solve the molecular mechanism of such a multi-tasking complex, dynamical structures with self, other proteins, and environment should be described. And to do this, interrogation within contexts increasingly close to native conditions is needed. Here we provide a perspective on how different experimental contexts have been used to study the exocyst, and those that could be used in the future. This review describes the structural breakthroughs on the isolated in vitro exocyst, followed by the use of membrane reconstitution assays for revealing in vitro exocyst functionality. Next, it moves to in situ cell contexts, reviewing imaging techniques that have been, and that ideally could be, used to look for near-native structure and organization dynamics. Finally, it looks at the exocyst structure in situ within evolutionary contexts, and the potential of structure prediction therein. From in vitro, to in situ, cross-context investigation of exocyst structure has begun, and will be critical for functional mechanism elucidation.
• dc.description.sponsorship This work was supported by the Spanish funding agency [PID2021-127773NB-I00 funded by MICIU/AEI/10.13039/501100011033/FEDER/UE, PRE 2019-088514 funded by MICIU/AEI/10.13039/501100011033 and by ‘FSE invierte en tu futuro’; the Unidad de Excelencia Maria de Maeztu [CEX2018-000792-M and CNS2022-135349 funded by MICIU/AEI/10.13039/501100011033/Unión Europea NextGenerationEU/PRTR]; and the Human Frontiers Science Program [RGP0017/2020]. S.M. is a recipient of a fellowship by the predoctoral program AGAUR-FI ajuts (2024 FI-3 00065) Joan Oró of the Secretariat of Universities and Research of the Department of Research and Universities of the Generalitat of Catalonia and the European Social Plus Fund.
• dc.format.mimetype application/pdf
• dc.identifier.citation Meek S, Hernandez AC, Oliva B, Gallego O. The exocyst in context. Biochem Soc Trans. 2024 Oct 30;52(5):2113-22. DOI: 10.1042/BST20231401
• dc.identifier.doi http://dx.doi.org/10.1042/BST20231401
• dc.identifier.issn 0300-5127
• dc.identifier.uri http://hdl.handle.net/10230/70016
• dc.language.iso eng
• dc.publisher Portland Press
• dc.relation.ispartof Biochem Soc Trans. 2024 Oct 30;52(5):2113-22
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-127773NB-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PRE2019-088514
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/CNS2022-135349
• dc.rights © 2024 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Evolutionary cell biology
• dc.subject.keyword Exocyst
• dc.subject.keyword Exocytosis
• dc.subject.keyword Structural cell biology
• dc.title The exocyst in context
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion