αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish

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• dc.contributor.author Han, Mitchell K. L.
• dc.contributor.author Hoijman, Esteban
• dc.contributor.author Nöel, Emily
• dc.contributor.author Garric, Laurence
• dc.contributor.author Bakkers, Jeroen
• dc.contributor.author de Rooij, Johan
• dc.date.accessioned 2024-01-22T06:50:28Z
• dc.date.available 2024-01-22T06:50:28Z
• dc.date.issued 2016
• dc.description.abstract Cadherin complexes mediate cell-cell adhesion and are crucial for embryonic development. Besides their structural function, cadherin complexes also transduce tension across the junction-actomyosin axis into proportional biochemical responses. Central to this mechanotransduction is the stretching of the cadherin-F-actin-linker α-catenin, which opens its central domain for binding to effectors such as vinculin. Mechanical unfolding of α-catenin leads to force-dependent reinforcement of cadherin-based junctions as studied in cell culture. The importance of cadherin mechanotransduction for embryonic development has not been studied yet. Here we used TALEN-mediated gene disruption to perturb endogenous αE-catenin in zebrafish development. Zygotic α-catenin mutants fail to maintain their epithelial barrier, resulting in tissue rupturing. We then specifically disrupted mechanotransduction, while maintaining cadherin adhesion, by expressing an αE-catenin construct in which the mechanosensitive domain was perturbed. Expression of either wild-type or mechano-defective α-catenin fully rescues barrier function in α-catenin mutants; however, expression of mechano-defective α-catenin also induces convergence and extension defects. Specifically, the polarization of cadherin-dependent, lamellipodia-driven cell migration of the lateral mesoderm was lost. These results indicate that cadherin mechanotransduction is crucial for proper zebrafish morphogenesis, and uncover one of the essential processes affected by its perturbation.
• dc.format.mimetype application/pdf
• dc.identifier.citation Han MKL, Hoijman E, Nöel E, Garric L, Bakkers J, de Rooij J. αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish. Biology Open. 2016 Oct;5(10):1461-72. DOI: 10.1242/bio.021378
• dc.identifier.doi http://dx.doi.org/10.1242/bio.021378
• dc.identifier.issn 2046-6390
• dc.identifier.uri http://hdl.handle.net/10230/58776
• dc.language.iso eng
• dc.publisher Company of Biologists
• dc.relation.ispartof Biology Open. 2016 Oct;5(10):1461-72
• dc.rights © 2016. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/3.0
• dc.subject.keyword Zebrafish
• dc.subject.keyword Cadherin mechanotransduction
• dc.subject.keyword Alpha-catenin
• dc.subject.keyword Convergent extension
• dc.subject.keyword Embryogenesis
• dc.title αE-catenin-dependent mechanotransduction is essential for proper convergent extension in zebrafish
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion