A novel translational control mechanism involving RNA structures within coding sequences

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• dc.contributor.author Jungfleisch, Jennifer, 1986-ca
• dc.contributor.author Nedialkova, Danny D.ca
• dc.contributor.author Dotu, Ivanca
• dc.contributor.author Sloan, Katherine E.ca
• dc.contributor.author Martínez Bosch, Neusca
• dc.contributor.author Brüning, Lukasca
• dc.contributor.author Raineri, Emanueleca
• dc.contributor.author Navarro Medrano, Pilarca
• dc.contributor.author Bohnsack, Markus T.ca
• dc.contributor.author Leidel, Sebastianca
• dc.contributor.author Díez Antón, Juana, 1962-ca
• dc.date.accessioned 2017-02-03T08:09:08Z
• dc.date.issued 2017ca
• dc.description.abstract The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here, we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and genome-wide ribosome profiling analyses, we show that this mechanism, initially derived from studies of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site; second, they are directly bound by Dhh1 with a specific binding distribution; and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a novel layer of translational control that involves RNA helicases and RNA folding within CDS providing novel opportunities for regulation of membrane and secretome proteins.
• dc.description.sponsorship This work was supported by the Spanish Ministry of Economy and Competitiveness through grant BFU 2013-44629-R and the “Maria de Maeztu” Programme for Units of Excellence in R&D (MDM-2014-0370). J.J. was supported by the grant 2012FI_B00574 from the Generalitat de Catalunya. This work was also supported by the Deutsche Forschungsgemeinschaft/n(SFB860 to M.T.B.), the Alexander von Humboldt foundation/n(to K.E.S. and M.T.B.), the Max Planck Society (to S.A.L.),/nthe Spanish Ministerio de Economia y Competividad/ISCIIIFEDER/n(PI14/00125 to P.N.), and the Generalitat de Catalunya/n(2014/SGR/143 to P.N.)
• dc.format.mimetype application/pdfca
• dc.identifier.citation Jungfleisch J, Nedialkova DD, Dotu I, Sloan KE, Martinez Bosch N, Brüning L et al. A novel translational control mechanism involving RNA structures within coding sequences. Genome Res. 2017 Jan;27(1):95-106. DOI: 10.1101/gr.209015.116ca
• dc.identifier.doi http://dx.doi.org/10.1101/gr.209015.116
• dc.identifier.issn 1088-9051ca
• dc.identifier.uri http://hdl.handle.net/10230/28041
• dc.language.iso engca
• dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)ca
• dc.relation.ispartof Genome Research. 2017 Jan;27(1):95-106
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2013-44629-R
• dc.rights Published originally by Cold Spring Harbor Laboratory Press at http://dx.doi.org/10.1101/gr.209015.116. Beginning six months from the full-issue publication date, articles are distributed under the Creative Commons Attribution-Non-Commercial 4.0 International License (CC-BY-NC), as described at http://creativecommons.org/licenses/by-nc/4.0/. This license permits non-commercial use, including reproduction, adaptation, and distribution of the article provided the original author and source are creditedca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
• dc.subject.other RNA
• dc.subject.other Genètica
• dc.subject.other Genomes
• dc.title A novel translational control mechanism involving RNA structures within coding sequencesca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca