The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α

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• dc.contributor.author Chandra, Vikash
• dc.contributor.author Ibrahim, Hazem
• dc.contributor.author Halliez, Clémentine
• dc.contributor.author Prasad, Rashmi B.
• dc.contributor.author Vecchio, Federica
• dc.contributor.author Dwivedi, Om Prakash
• dc.contributor.author Kvist, Jouni
• dc.contributor.author Balboa, Diego
• dc.contributor.author Saarimäki-Vire, Jonna
• dc.contributor.author Montaser, Hossam
• dc.contributor.author Barsby, Tom
• dc.contributor.author Lithovius, Väinö
• dc.contributor.author Artner, Isabella
• dc.contributor.author Gopalakrishnan, Swetha
• dc.contributor.author Groop, Leif
• dc.contributor.author Mallone, Roberto
• dc.contributor.author Eizirik, Décio L.
• dc.contributor.author Otonkoski, Timo
• dc.date.accessioned 2023-01-24T07:20:00Z
• dc.date.available 2023-01-24T07:20:00Z
• dc.date.issued 2022
• dc.description.abstract Type 1 diabetes (T1D) is an autoimmune disease that results in the destruction of insulin producing pancreatic β-cells. One of the genes associated with T1D is TYK2, which encodes a Janus kinase with critical roles in type-Ι interferon (IFN-Ι) mediated intracellular signalling. To study the role of TYK2 in β-cell development and response to IFNα, we generated TYK2 knockout human iPSCs and directed them into the pancreatic endocrine lineage. Here we show that loss of TYK2 compromises the emergence of endocrine precursors by regulating KRAS expression, while mature stem cell-islets (SC-islets) function is not affected. In the SC-islets, the loss or inhibition of TYK2 prevents IFNα-induced antigen processing and presentation, including MHC Class Ι and Class ΙΙ expression, enhancing their survival against CD8+ T-cell cytotoxicity. These results identify an unsuspected role for TYK2 in β-cell development and support TYK2 inhibition in adult β-cells as a potent therapeutic target to halt T1D progression.
• dc.description.sponsorship We gratefully acknowledge Dr. Fatoumata Samassa for help with cytotoxicity assays. We thank Jarkko Ustinov for the insulin and c-peptide ELISA measurements. S. Eurola, H. Grym, and A. Laitinen are thanked for expert technical support. We thank FIMM Single Cell Analytics unit (supported by HiLIFE and Biocentre Finland) for single cell RNA sequencing services. We want to acknowledge the participants and investigators of the FinnGen study. T.O. acknowledges the funding provided by the Academy of Finland (MetaStem Center of Excellence grant 312437), the Novo Nordisk Foundation and the Sigrid Juselius Foundation. R.M. acknowledges the support of the Agence Nationale de la Recherche (ANR-19-CE15-0014-01) and the Fondation pour la Recherche Medicale (EQU20193007831). C.H. was funded by an Année Recherche fellowship of the Paris Saclay University. F.V. was funded by an international PhD fellowship of the IdEx Université de Paris. R.B.P. acknowledges the funding by Hjelt foundation, Crafoord foundation (2020089) and Swedish Research Council (2021-02623). D.L.E. acknowledges the support of grants from the Welbio-FNRS (Fonds National de la Recherche Scientifique; WELBIO-CR-2019C-04), Belgium; the Innovate2CureType1—Dutch Diabetes Research Foundation (DDRF), Holland; the Juvenile Diabetes Foundation (JDRF; 2-SRA-2019-834-S-B); the NIH (HIRN-CBDS) grant U01 DK127786, USA. D.L.E., T.O., and R.M. acknowledge support from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreements No 115797 (INNODIA) and 945268 (INNODIA HARVEST), supported by the European Union’s Horizon 2020 research and innovation programme. These Joint Undertakings receive support from the Union’s Horizon 2020 research and innovation programme and “EFPIA”, “JDRF” and “The Leona M. and Harry B. Helmsley Charitable Trust”.
• dc.format.mimetype application/pdf
• dc.identifier.citation Chandra V, Ibrahim H, Halliez C, Prasad RB, Vecchio F, Dwivedi OP, Kvist J, Balboa D, Saarimäki-Vire J, Montaser H, Barsby T, Lithovius V, Artner I, Gopalakrishnan S, Groop L, Mallone R, Eizirik DL, Otonkoski T. The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α. Nat Commun. 2022 Oct 26;13(1):6363. DOI: 10.1038/s41467-022-34069-z
• dc.identifier.doi http://dx.doi.org/10.1038/s41467-022-34069-z
• dc.identifier.issn 2041-1723
• dc.identifier.uri http://hdl.handle.net/10230/55413
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Nat Commun. 2022 Oct 26;13(1):6363
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/115797
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/945268
• dc.rights © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Cellular immunity
• dc.subject.keyword Disease model
• dc.subject.keyword Stem-cell differentiation
• dc.subject.keyword Type 1 diabetes
• dc.title The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion