Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Espadas, Guadalupeca
• dc.contributor.author Borràs, Evaca
• dc.contributor.author Chiva, Cristinaca
• dc.contributor.author Sabidó Aguadé, Eduard, 1981-ca
• dc.date.accessioned 2018-06-07T09:47:32Z
• dc.date.available 2018-06-07T09:47:32Z
• dc.date.issued 2017
• dc.description.abstract One of the major additions in MS technology has been the irruption of the Orbitrap mass analyzer, which has boosted the proteomics analyses of biological complex samples since its introduction. Here, we took advantage of the capabilities of the new Orbitrap Fusion Lumos Tribrid mass spectrometer to assess the performance of different data-dependent acquisition methods for the identification and quantitation of peptides and phosphopeptides in single-shot analysis of human whole cell lysates. Our study explored the capabilities of tri-hibrid mass spectrometers for (phospho-) peptide identification and quantitation using different gradient lengths, sample amounts, and combinations of different peptide fragmentation types and mass analyzers. Moreover, the acquisition of the same complex sample with different acquisition methods resulted in the generation of a dataset to be used as a reference for further analyses, and a starting point for future optimizations in particular applications.
• dc.description.sponsorship The CRG/UPF Proteomics Unit is part of the “Plataforma de Recursos Biomoleculares y Bioinformáticos (ProteoRed)” supported by grant PT13/0001 of ISCIII and Spanish Ministry of Economy and Competitiveness. We acknowledge support of the Spanish Ministry of Economy and Competitiveness, “Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016” (CTQ2016‐80364‐P) “Centro de Excelencia Severo Ochoa 2013-2017” (SEV‐2012‐0208), and from “Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya” (2014SGR678).
• dc.format.mimetype application/pdf
• dc.identifier.citation Espadas G, Borràs E, Chiva C, Sabidó E. Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer. Proteomics. 2017 May;17(9):1600416. DOI: 10.1002/pmic.201600416
• dc.identifier.doi http://dx.doi.org/10.1002/pmic.201600416
• dc.identifier.issn 1615-9853
• dc.identifier.uri http://hdl.handle.net/10230/34853
• dc.language.iso eng
• dc.publisher Wileyca
• dc.relation.ispartof Proteomics. 2017 May;17(9):1600416
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/CTQ2016-80364-P
• dc.rights This is the peer reviewed version of the following article: Espadas G, Borràs E, Chiva C, Sabidó E. Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer. Proteomics. 2017 May;17(9):1600416, which has been published in final form at http://dx.doi.org/10.1002/pmic.201600416. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.keyword Mass spectrometry
• dc.subject.keyword Phosphorylation
• dc.subject.keyword Protein identification
• dc.subject.keyword Protein quantitation
• dc.title Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometerca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion