Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position

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• dc.contributor.author Lang, Michaelca
• dc.contributor.author Hadzhiev, Yavorca
• dc.contributor.author Siegel, Nicolca
• dc.contributor.author Amemiya, Chris Tca
• dc.contributor.author Parada, Carolinaca
• dc.contributor.author Strähle, Uweca
• dc.contributor.author Becker, May-Brittca
• dc.contributor.author Müller, Ferencca
• dc.contributor.author Meyer, Axelca
• dc.date.accessioned 2014-12-22T13:00:24Z
• dc.date.available 2014-12-22T13:00:24Z
• dc.date.issued 2010ca
• dc.description.abstract Background: The modern coelacanth (Latimeria) is the extant taxon of a basal sarcopterygian lineage and sister group to tetrapods. Apart from certain apomorphic traits, its morphology is characterized by a high degree of retention of ancestral vertebrate structures and little morphological change. An insight into the molecular evolution that may explain the unchanged character of Latimeria morphology requires the analysis of the expression patterns of developmental regulator genes and their cis-regulatory modules (CRMs). Results: We describe the comparative and functional analysis of the sonic hedgehog (shh) genomic region of Latimeria menadoensis. Several putative enhancers in the Latimeria shh locus have been identified by comparisons to sarcopterygian and actinopterygian extant species. Specific sequence conservation with all known actinopterygian enhancer elements has been detected. However, these elements are selectively missing in more recently diverged actinopterygian and sarcopterygian species. The functionality of the putative Latimeria enhancers was confirmed by reporter gene expression analysis in transient transgenic zebrafish and chick embryos./n/nConclusions: Latimeria shh CRMs represent the ancestral set of enhancers that have emerged before the split of lobe-finned and ray-finned fishes. In contrast to lineage-specific losses and differentiations in more derived lineages, Latimeria shh enhancers reveal low levels of sequence diversification. High overall sequence conservation of shh conserved noncoding elements (CNE) is consistent with the general trend of high levels of conservation of noncoding DNA in the slowly evolving Latimeria genome.
• dc.description.abstract This project was supported by EUTRACC and Transcode of the EU Framework programmes (contracts 511990 and LSGH CT 2006037445 and by the Deutsche Forschungsgemeinschaft (MU1768/2)). U. S. was supported by EC IPs ZF-Models and Eutracc and the Helmholtz Association. C. T. A. was supported by grants from the National Science Foundation (IBN-0321461, MCB-0719558). A. M. was supported by grants from the Deutsche Forschungsgemeinschaft.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Lang M, Hadzhiev Y, Siegel N, Amemiya CT, Parada C, Strähle U et al. Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic position. EvoDevo. 2010 Nov;1:11. DOI: 10.1186/2041-9139-1-11ca
• dc.identifier.doi http://dx.doi.org/10.1186/2041-9139-1-11
• dc.identifier.issn 2041-9139ca
• dc.identifier.uri http://hdl.handle.net/10230/23029
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof EvoDevo. 2010 Nov;1:11
• dc.rights © 2010 Lang et al; licensee BioMed Central Ltd. /nThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.other Vertebrats
• dc.subject.other Gens
• dc.title Conservation of shh cis-regulatory architecture of the coelacanth is consistent with its ancestral phylogenetic positionca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca