Association of porcine Swine Leukocyte Antigen (SLA) haplotypes with B- and T-cell immune response to Foot-and-Mouth Disease Virus (FMDV) peptides

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author de León, Patricia
• dc.contributor.author Cañas Arranz, Rodrigo
• dc.contributor.author Saez, Yago
• dc.contributor.author Forner, Mar, 1980-
• dc.contributor.author Defaus, Sira
• dc.contributor.author Cuadra, Dolores
• dc.contributor.author Bustos, María J.
• dc.contributor.author Torres, Elisa
• dc.contributor.author Andreu Martínez, David
• dc.contributor.author Blanco, Esther
• dc.contributor.author Sobrino, Francisco
• dc.contributor.author Hammer, Sabine E.
• dc.date.accessioned 2020-10-08T06:16:04Z
• dc.date.available 2020-10-08T06:16:04Z
• dc.date.issued 2020
• dc.description.abstract Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic B-cell site in VP1 protein, are covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies and IFN-γ-producing cells in pigs. To address the contribution of the highly polymorphic nature of the porcine MHC (SLA, swine leukocyte antigen) on the immunogenicity of B2T dendrimers, low-resolution (Lr) haplotyping was performed. We looked for possible correlations between particular Lr haplotypes with neutralizing antibody and T-cell responses induced by B2T peptides. In this study, 63 pigs immunized with B2T dendrimers and 10 non-immunized (control) animals are analyzed. The results reveal a robust significant correlation between SLA class-II Lr haplotypes and the T-cell response. Similar correlations of T-cell response with SLA class-I Lr haplotypes, and between B-cell antibody response and SLA class-I and SLA class-II Lr haplotypes, were only found when the sample was reduced to animals with Lr haplotypes represented more than once. These results support the contribution of SLA class-II restricted T-cells to the magnitude of the T-cell response and to the antibody response evoked by the B2T dendrimers, being of potential value for peptide vaccine design against FMDV.
• dc.format.mimetype application/pdf
• dc.identifier.citation de León P, Cañas-Arranz R, Saez Y, Forner M, Defaus S, Cuadra D, Bustos MJ, Torres E, Andreu D, Blanco E, Sobrino F, Hammer SE. Association of porcine Swine Leukocyte Antigen (SLA) haplotypes with B- and T-cell immune response to Foot-and-Mouth Disease Virus (FMDV) peptides. Vaccines (Basel). 2020; 8(3):E513. DOI: 10.3390/vaccines8030513
• dc.identifier.doi http://dx.doi.org/10.3390/vaccines8030513
• dc.identifier.issn 2076-393X
• dc.identifier.uri http://hdl.handle.net/10230/45424
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Vaccines (Basel). 2020; 8(3):E513
• dc.rights © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword SLA polymorphism
• dc.subject.keyword Adaptive immunity
• dc.subject.keyword Dendrimer peptide
• dc.subject.keyword Foot-and-mouth disease virus
• dc.subject.keyword Protection
• dc.subject.keyword Swine
• dc.subject.keyword Swine leukocyte antigen
• dc.subject.keyword Vaccine
• dc.title Association of porcine Swine Leukocyte Antigen (SLA) haplotypes with B- and T-cell immune response to Foot-and-Mouth Disease Virus (FMDV) peptides
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion