Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns

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• dc.contributor.author Hümmer, Stefan
• dc.contributor.author Borao Arriazu, Sonia, 1993-
• dc.contributor.author Guerra Moreno, Ángel
• dc.contributor.author Cozzuto, Luca
• dc.contributor.author Hidalgo Hernando, Elena
• dc.contributor.author Ayté del Olmo, José
• dc.date.accessioned 2021-12-14T06:54:57Z
• dc.date.available 2021-12-14T06:54:57Z
• dc.date.issued 2021
• dc.description.abstract Splicing of mRNA precursors is essential in the regulation of gene expression. U2AF65 recognizes the poly-pyrimidine tract and helps in the recognition of the branch point. Inactivation of fission yeast U2AF65 (Prp2) blocks splicing of most, but not all, pre-mRNAs, for reasons that are not understood. Here, we have determined genome-wide the splicing efficiency of fission yeast cells as they progress into synchronous meiosis in the presence or absence of functional Prp2. Our data indicate that in addition to the splicing elements at the 3' end of any intron, the nucleotides immediately upstream the intron will determine whether Prp2 is required or dispensable for splicing. By changing those nucleotides in any given intron, we regulate its Prp2 dependency. Our results suggest a model in which Prp2 is required for the coordinated recognition of both intronic ends, placing Prp2 as a key regulatory element in the determination of the exon-intron boundaries.
• dc.description.sponsorship This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (MINECO) (BFU2015-66347-P and BFU2018-PGC2018-097248-B-I00), PLAN E and Feder, MEIONet (BFU2015-71786-REDT), and Unidad de Excelencia María de Maeztu (CEX2018-000792-M). E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Hümmer S, Borao S, Guerra-Moreno A, Cozzuto L, Hidalgo E, Ayté J. Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns. Cell Rep. 2021;37(4):109893. DOI: 10.1016/j.celrep.2021.109893
• dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2021.109893
• dc.identifier.issn 2211-1247
• dc.identifier.uri http://hdl.handle.net/10230/49191
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Cell Rep. 2021;37(4):109893
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347-P
• dc.rights © 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword 5′ splicing site
• dc.subject.keyword Prp2
• dc.subject.keyword U2AF65
• dc.subject.keyword Fission yeast
• dc.subject.keyword Meiosis
• dc.subject.keyword Splicing
• dc.title Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion