Protective effect of hydroxytyrosol and tyrosol metabolites in LPS-induced vascular barrier derangement in vitro

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• dc.contributor.author Zodio, Sonia
• dc.contributor.author Serreli, Gabriele
• dc.contributor.author Melis, Maria Paola
• dc.contributor.author Franchi, Benedetta
• dc.contributor.author Boronat Rigol, Anna, 1990-
• dc.contributor.author Torre Fornell, Rafael de la
• dc.contributor.author Deiana, Monica
• dc.date.accessioned 2024-06-11T06:13:39Z
• dc.date.available 2024-06-11T06:13:39Z
• dc.date.issued 2024
• dc.description.abstract Introduction: The maintenance of endothelial barrier function is essential for vasal homeostasis and prevention of cardiovascular diseases. Among the toxic stimuli involved in the initiation of atherosclerotic lesions, Gram negative lipopolysaccharide (LPS) has been reported to be able to trigger endothelial dysfunction, through the alteration of barrier permeability and inflammatory response. Hydroxytyrosol (HT) and tyrosol (Tyr), the major phenolic compounds of extra virgin olive oil (EVOO), as wells as their circulating sulphated and glucuronidated metabolites have been shown to exert anti-inflammatory effects at endothelial level. Methods: In this study we investigated the protective effects of HT and Tyr metabolites on LPS-induced alteration of permeability in Human Umbilical Vein Endothelial Cells (HUVEC) monolayers and examined underlying signaling pathways, focusing on tight junction (TJ) proteins, mitogen-activated protein kinase (MAPK) and NOD-, LRR-and pyrin domain-containing protein 3 (NLRP3) inflammasome activation. Results: It was shown that LPS-increased permeability in HUVEC cells was due to the alteration of TJ protein level, following the activation of MAPK and NLRP3. HT and Tyr sulphated and glucuronidated metabolites were able to limit the effects exerted by LPS, acting as signaling molecules with an efficacy comparable to that of their precursors HT and Tyr. Discussion: The obtained results add a further piece to the understanding of HT and Tyr metabolites mechanisms of action in vascular protection.
• dc.format.mimetype application/pdf
• dc.identifier.citation Zodio S, Serreli G, Melis MP, Franchi B, Boronat A, de la Torre R, et al. Protective effect of hydroxytyrosol and tyrosol metabolites in LPS-induced vascular barrier derangement in vitro. Front Nutr. 2024 Apr 19;11:1350378. DOI: 10.3389/fnut.2024.1350378
• dc.identifier.doi http://dx.doi.org/10.3389/fnut.2024.1350378
• dc.identifier.issn 2296-861X
• dc.identifier.uri http://hdl.handle.net/10230/60425
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Front Nutr. 2024 Apr 19;11:1350378
• dc.rights © 2024 Zodio, Serreli, Melis, Franchi, Boronat, de la Torre and Deiana. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Endothelial dysfunction
• dc.subject.keyword Extra virgin olive oil
• dc.subject.keyword Inflammation
• dc.subject.keyword Phenolic compounds
• dc.subject.keyword Tight junctions
• dc.title Protective effect of hydroxytyrosol and tyrosol metabolites in LPS-induced vascular barrier derangement in vitro
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion