Lack of tailless leads to an increase in expression variability in Drosophila embryos

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• dc.contributor.author Janssens, Hilde
• dc.contributor.author Crombach, Anton
• dc.contributor.author Wotton, Karl R.
• dc.contributor.author Cicin Sain, Damjan
• dc.contributor.author Surkova, Svetlana
• dc.contributor.author Lim, Chea Lu
• dc.contributor.author Samsonova, Maria
• dc.contributor.author Akam, Michael
• dc.contributor.author Jaeger, Johannes, 1973-
• dc.date.accessioned 2024-01-29T07:22:19Z
• dc.date.available 2024-01-29T07:22:19Z
• dc.date.issued 2013
• dc.description.abstract Developmental processes are robust, or canalised: dynamic patterns of gene expression across space and time are regulated reliably and precisely in the presence of genetic and environmental perturbations. It remains unclear whether canalisation relies on specific regulatory factors (such as heat-shock proteins), or whether it is based on more general redundancy and distributed robustness at the network level. The latter explanation implies that mutations in many regulatory factors should exhibit loss of canalisation. Here, we present a quantitative characterisation of segmentation gene expression patterns in mutants of the terminal gap gene tailless (tll) in Drosophila melanogaster. Our analysis provides new insights into the dynamic mechanisms underlying gap gene regulation, and reveals significantly increased variability of gene expression in the mutant compared to the wild-type background. We show that both position and timing of posterior segmentation gene expression domains vary strongly from embryo-to-embryo in tll mutants. This variability must be caused by a vulnerability in the regulatory system which is hidden or buffered in the wild-type, but becomes uncovered by the deletion of tll. Our analysis provides evidence that loss of canalisation in mutants could be more widespread than previously thought.
• dc.description.sponsorship We thank John Reinitz and Stephen Small for providing fly stocks. We acknowledge help and support from the confocal imaging facility at the Department of Zoology in Cambridge, and the Advanced Microscopy Core Facility at the Centre for Genomic Regulation (CRG). We thank Konstantin Kozlov for extensive and helpful support concerning his BREReA software. Jordi Casanova provided crucial bits of inspiration and insight. We acknowledge one of the anonymous reviewers for suggesting the possible mechanism of stripe 7 regulation in mutants. Work in Cambridge and Barcelona was funded by the UK Biotechnology and Biological Sciences Research Council (grant number BB/D00513), by the MEC-EMBL agreement for the EMBL/CRG Research Unit in Systems Biology, and by Grant BFU2009-10184 of the Spanish Ministry of Science and Innovation (MICINN). The Samsonova group in St. Petersburg was funded by a state scientific contract with the Russian Ministry of Education and Science (N 14.740.11.0166), and the Russian Foundation for Basic Research (grants 10-01-00627-a, 11-04-01162-a and 11-01-00573-a).
• dc.format.mimetype application/pdf
• dc.identifier.citation Janssens H, Crombach A, Wotton KR, Cicin-Sain D, Surkova S, Lim CL, et al. Lack of tailless leads to an increase in expression variability in Drosophila embryos. Developmental Biology. 2013 May 01;377(1):305-17. DOI: 10.1016/j.ydbio.2013.01.010
• dc.identifier.doi http://dx.doi.org/10.1016/j.ydbio.2013.01.010
• dc.identifier.issn 0012-1606
• dc.identifier.uri http://hdl.handle.net/10230/58851
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Developmental Biology. 2013 May 01;377(1):305-17
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-10184
• dc.rights Copyright © 2013 Elsevier Inc. This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/3.0/
• dc.subject.keyword Drosophila embryogenesis
• dc.subject.keyword Segmentation gene network
• dc.subject.keyword Quantitative expression analysis
• dc.subject.keyword Pattern formation
• dc.subject.keyword Robustness/canalisation
• dc.subject.keyword Genetic capacitance
• dc.title Lack of tailless leads to an increase in expression variability in Drosophila embryos
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion