Gene expression profiles controlled by the alternative splicing factor nova2 in endothelial cells

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• dc.contributor.author Belloni, Elisa
• dc.contributor.author Di Matteo, Anna
• dc.contributor.author Pradella, Davide
• dc.contributor.author Vacca, Margherita
• dc.contributor.author Wyatt, Christopher Douglas Robert, 1988-
• dc.contributor.author Alfieri, Roberta
• dc.contributor.author Maffia, Antonio
• dc.contributor.author Sabbioneda, Simone
• dc.contributor.author Ghigna, Claudia
• dc.date.accessioned 2020-04-14T07:15:19Z
• dc.date.available 2020-04-14T07:15:19Z
• dc.date.issued 2019
• dc.description.abstract Alternative splicing (AS) plays an important role in expanding the complexity of the human genome through the production of specialized proteins regulating organ development and physiological functions, as well as contributing to several pathological conditions. How AS programs impact on the signaling pathways controlling endothelial cell (EC) functions and vascular development is largely unknown. Here we identified, through RNA-seq, changes in mRNA steady-state levels in ECs caused by the neuro-oncological ventral antigen 2 (Nova2), a key AS regulator of the vascular morphogenesis. Bioinformatics analyses identified significant enrichment for genes regulated by peroxisome proliferator-activated receptor-gamma (Ppar-γ) and E2F1 transcription factors. We also showed that Nova2 in ECs controlled the AS profiles of Ppar-γ and E2F dimerization partner 2 (Tfdp2), thus generating different protein isoforms with distinct function (Ppar-γ) or subcellular localization (Tfdp2). Collectively, our results supported a mechanism whereby Nova2 integrated splicing decisions in order to regulate Ppar-γ and E2F1 activities. Our data added a layer to the sequential series of events controlled by Nova2 in ECs to orchestrate vascular biology.
• dc.format.mimetype application/pdf
• dc.identifier.citation Belloni E, Di Matteo A, Pradella D, Vacca M, Wyatt CDR, Alfieri R, Maffia A, Sabbioneda S, Ghigna C. Gene expression profiles controlled by the alternative splicing factor nova2 in endothelial cells. Cells. 2019; 8(12). pii: E1498. DOI: 10.3390/cells8121498
• dc.identifier.doi http://dx.doi.org/10.3390/cells8121498
• dc.identifier.issn 2073-4409
• dc.identifier.uri http://hdl.handle.net/10230/44204
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Cells. 2019; 8(12). pii: E1498
• dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Nova2
• dc.subject.keyword Alternative splicing
• dc.subject.keyword Angiogenesis
• dc.subject.keyword Post-transcriptional regulation
• dc.subject.keyword Vascular development
• dc.title Gene expression profiles controlled by the alternative splicing factor nova2 in endothelial cells
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion