The human sperm basal body is a complex centrosome important for embryo preimplantation development
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- dc.contributor.author Amargant i Riera, Farners, 1990-
- dc.contributor.author Pujol, Aïda
- dc.contributor.author Ferrer-Vaquer, Anna
- dc.contributor.author Durban, Mercè
- dc.contributor.author Martínez, Meritxell
- dc.contributor.author Vassena, Rita
- dc.contributor.author Vernos, Isabelle, 1959-
- dc.date.accessioned 2022-01-26T12:45:43Z
- dc.date.available 2022-01-26T12:45:43Z
- dc.date.issued 2021
- dc.description.abstract The mechanism of conversion of the human sperm basal body to a centrosome after fertilization, and its role in supporting human early embryogenesis, has not been directly addressed so far. Using proteomics and immunofluorescence studies, we show here that the human zygote inherits a basal body enriched with centrosomal proteins from the sperm, establishing the first functional centrosome of the new organism. Injection of human sperm tails containing the basal body into human oocytes followed by parthenogenetic activation, showed that the centrosome contributes to the robustness of the early cell divisions, increasing the probability of parthenotes reaching the compaction stage. In the absence of the sperm-derived centrosome, pericentriolar material (PCM) components stored in the oocyte can form de novo structures after genome activation, suggesting a tight PCM expression control in zygotes. Our results reveal that the sperm basal body is a complex organelle which converts to a centrosome after fertilization, ensuring the early steps of embryogenesis and successful compaction. However, more experiments are needed to elucidate the exact molecular mechanisms of centrosome inheritance in humans.
- dc.description.sponsorship F.A. was supported by a fellowship from the Agency for Management of University and Research Grants from the Government of Catalonia (AGAUR-2014 DI 065). Work in the Vernos lab was supported by the Spanish Ministry of Economy and Competitiveness (BFU2015-68726-P), the Ministry of Science, Innovation and Universities (PGC2018-096976-B-I00) and intramural funds from the Centre for Genomic Regulation. Intramural funding from Clínica EUGIN partially supported the study. We acknowledge the support of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the EMBL partnership, the Spanish Ministry of Economy and Competitiveness, Centro de Excelencia ‘Severo Ochoa’ and the CERCA programme/Generalitat de Catalunya
- dc.format.mimetype application/pdf
- dc.identifier.citation Amargant F, Pujol A, Ferrer-Vaquer A, Durban M, Martínez M, Vassena R et al. The human sperm basal body is a complex centrosome important for embryo preimplantation development. Mol Hum Reprod. 2021 Nov 2;27(11):gaab062. DOI: 10.1093/molehr/gaab062
- dc.identifier.doi http://dx.doi.org/10.1093/molehr/gaab062
- dc.identifier.issn 1460-2407
- dc.identifier.uri http://hdl.handle.net/10230/52331
- dc.language.iso eng
- dc.publisher Oxford University Press
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P
- dc.rights © Farners Amargant et al. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by-nc/4.0/
- dc.subject.other Embriologia humana
- dc.subject.other Semen
- dc.title The human sperm basal body is a complex centrosome important for embryo preimplantation development
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion