Nitro-oxidative stress after neuronal ischemia induces protein nitrotyrosination and cell death
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Tajes Orduña, Martaca
- dc.contributor.author Ill-Raga, Gerard, 1982-ca
- dc.contributor.author Palomer, Ernestca
- dc.contributor.author Ramos Fernández, Eva, 1984-ca
- dc.contributor.author Guix Ràfols, Francesc Xavierca
- dc.contributor.author Bosch Morató, Mònica, 1986-ca
- dc.contributor.author Guivernau Almazán, Biuse, 1988-ca
- dc.contributor.author Jiménez Conde, Jordica
- dc.contributor.author Ois Santiago, Angel Javierca
- dc.contributor.author Pérez Asensio, Fernandoca
- dc.contributor.author Reyes Navarro, Marioca
- dc.contributor.author Caballo, Carolinaca
- dc.contributor.author Galán, Ana M.ca
- dc.contributor.author Alameda Quitllet, Franciscoca
- dc.contributor.author Escolar, Ginèsca
- dc.contributor.author Opazo, Carlos M.ca
- dc.contributor.author Planas, Annaca
- dc.contributor.author Roquer, Jaumeca
- dc.contributor.author Valverde, M. A. (Miguel Ángel), 1963-ca
- dc.contributor.author Muñoz López, Francisco José, 1964-ca
- dc.date.accessioned 2015-12-02T19:27:58Z
- dc.date.available 2015-12-02T19:27:58Z
- dc.date.issued 2013
- dc.description.abstract Ischemic stroke is an acute vascular event that obstructs blood supply to the brain, producing irreversible damage that affects neurons but also glial and brain vessel cells. Immediately after the stroke, the ischemic tissue produces nitric oxide (NO) to recover blood perfusion but also produces superoxide anion. These compounds interact, producing peroxynitrite, which irreversibly nitrates protein tyrosines. The present study measured NO production in a human neuroblastoma (SH-SY5Y), a murine glial (BV2), a human endothelial cell line (HUVEC), and in primary cultures of human cerebral myocytes (HC-VSMCs) after experimental ischemia in vitro. Neuronal, endothelial, and inducible NO synthase (NOS) expression was also studied up to 24 h after ischemia, showing a different time course depending on the NOS type and the cells studied. Finally, we carried out cell viability experiments on SH-SY5Y cells with H2O2, a prooxidant agent, and with a NO donor to mimic ischemic conditions. We found that both compounds were highly toxic when they interacted, producing peroxynitrite. We obtained similar results when all cells were challenged with peroxynitrite. Our data suggest that peroxynitrite induces cell death and is a very harmful agent in brain ischemia.ca
- dc.format.mimetype application/pdfca
- dc.identifier.citation Tajes M, Ill-Raga G, Palomer E, Ramos-Fernández E, Guix FX, Bosch-Morató M et al. Nitro-oxidative stress after neuronal ischemia induces protein nitrotyrosination and cell death. Oxid Med Cell Longev. 2013;2013:826143. DOI: 10.1155/2013/826143ca
- dc.identifier.doi http://dx.doi.org/10.1155/2013/826143
- dc.identifier.issn 1942-0900
- dc.identifier.uri http://hdl.handle.net/10230/25318
- dc.language.iso engca
- dc.publisher Hindawica
- dc.relation.ispartof Oxidative medicine and cellular longevity. 2013; 2013: 826143
- dc.rights © 2013 Tajes M et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Estrès oxidatiuca
- dc.subject.other Proteïnes -- Metabolismeca
- dc.title Nitro-oxidative stress after neuronal ischemia induces protein nitrotyrosination and cell deathca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca