DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Gerra, Maria Carla
- dc.contributor.author Carnevali, Davide
- dc.contributor.author Ossola, Paolo
- dc.contributor.author González-Villar, Alberto
- dc.contributor.author Pedersen, Inge Søkilde
- dc.contributor.author Triñanes, Yolanda
- dc.contributor.author Donnini, Claudia
- dc.contributor.author Manfredini, Matteo
- dc.contributor.author Arendt-Nielsen, Lars
- dc.contributor.author Carrillo-de-la-Peña, Maria Teresa
- dc.date.accessioned 2022-03-15T07:23:10Z
- dc.date.available 2022-03-15T07:23:10Z
- dc.date.issued 2021
- dc.description.abstract Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the GCSAML region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to GCSAML survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; GRM2 methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the GRM2 region were significantly associated with FM risk. Our study encourages better exploration of GCSAML and GRM2 functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain.
- dc.description.sponsorship This research was funded by the Spanish Government Funding (Ministerio de Economía y Competitividad: grant PSI2013-45818-R). MCG and LAN are part of the Center for Neuroplasticity and Pain (CNAP) which is supported by the Danish National Research Foundation (DNRF121)
- dc.format.mimetype application/pdf
- dc.identifier.citation Gerra MC, Carnevali D, Ossola P, González-Villar A, Pedersen IS, Triñanes Y et al. DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization. J Clin Med. 2021 Oct 27;10(21):4992. DOI: 10.3390/jcm10214992
- dc.identifier.doi http://dx.doi.org/10.3390/jcm10214992
- dc.identifier.issn 2077-0383
- dc.identifier.uri http://hdl.handle.net/10230/52698
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/PSI2013-45818-R
- dc.rights © 2021 by Maria Carla Gerra et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/)
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri https://creativecommons.org/licenses/by/4.0/
- dc.subject.other Fibromiàlgia
- dc.subject.other DNA -- Metilació
- dc.subject.other Sistema immunològic
- dc.title DNA methylation changes in fibromyalgia suggest the role of the immune-inflammatory response and central sensitization
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion