Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytes

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• dc.contributor.author Paco, Soniaca
• dc.contributor.author Hummel, Manuelaca
• dc.contributor.author Plá, Virginiaca
• dc.contributor.author Sumoy Van Dyck, Lauroca
• dc.contributor.author Aguado, Fernandoca
• dc.date.accessioned 2016-06-01T14:13:49Z
• dc.date.available 2016-06-01T14:13:49Z
• dc.date.issued 2016
• dc.description.abstract BACKGROUND: cAMP signaling produces dramatic changes in astrocyte morphology and physiology. However, its involvement in phenotype acquisition and the transcriptionally mediated mechanisms of action are largely unknown. RESULTS: Here we analyzed the global transcriptome of cultured astroglial cells incubated with activators of cAMP pathways. A bulk of astroglial transcripts, 6221 annotated genes, were differentially regulated by cAMP signaling. cAMP analogs strongly upregulated genes involved in typical functions of mature astrocytes, such as homeostatic control, metabolic and structural support to neurons, antioxidant defense and communication, whereas they downregulated a considerable number of proliferating and immaturity-related transcripts. Moreover, numerous genes typically activated in reactive cells, such as scar components and immunological mediators, were repressed by cAMP. GSEA analysis contrasting gene expression profiles with transcriptome signatures of acutely isolated astrocytes and in situ evaluation of protein levels in these cells showed that cAMP signaling conferred mature and in vivo-like transcriptional features to cultured astrocytes. CONCLUSIONS: These results indicate that cAMP signaling is a key pathway promoting astrocyte maturation and restricting their developmental and activation features. Therefore, a positive modulation of cAMP signaling may promote the normal state of differentiated astrocytes and favor the protection and function of neuronal networks.ca
• dc.description.sponsorship This work was supported by grants from Spanish Ministry of Economy and Competitiveness (BFU2010-22132 and BFU2013-48822-R) and from Catalonian Government (2014SGR-01178) to F.A
• dc.format.mimetype application/pdfca
• dc.identifier.citation Paco S, Hummel M, Plá V, Sumoy L, Aguado F. Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytes. BMC Genomics. 2016; 17(1): 304. DOI 10.1186/s12864-016-2623-4ca
• dc.identifier.doi http://dx.doi.org/10.1186/s12864-016-2623-4
• dc.identifier.issn 1471-2164
• dc.identifier.uri http://hdl.handle.net/10230/26795
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof BMC Genomics. 2016; 17(1): 304
• dc.rights © 2016 Paco et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
• dc.subject.keyword Antioxidant defense
• dc.subject.keyword Astrocytes
• dc.subject.keyword Brain
• dc.subject.keyword Differentiation
• dc.subject.keyword NR2C
• dc.subject.keyword Reactive glia
• dc.subject.keyword Transcriptomic
• dc.subject.keyword cAMP
• dc.subject.other Antioxidantsca
• dc.title Cyclic AMP signaling restricts activation and promotes maturation and antioxidant defenses in astrocytesca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca