Distinct properties and functions of CTCF revealed by a rapidly inducible degron system

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• dc.contributor.author Luan, Jing
• dc.contributor.author Xiang, Guanjue
• dc.contributor.author Gómez García, Pablo
• dc.contributor.author Tome, Jacob M.
• dc.contributor.author Zhang, Zhe
• dc.contributor.author Vermut, Marit W.
• dc.contributor.author Zhang, Haoyue
• dc.contributor.author Huang, Anran
• dc.contributor.author Keller, Cheryl A.
• dc.contributor.author Giardine, Belinda M.
• dc.contributor.author Zhang, Yu
• dc.contributor.author Lan, Yemin
• dc.contributor.author Lis, John T.
• dc.contributor.author Lakadamyali, Melike
• dc.contributor.author Hardison, Ross C.
• dc.contributor.author Blobel, Gerd A.
• dc.date.accessioned 2022-04-27T10:57:43Z
• dc.date.available 2022-04-27T10:57:43Z
• dc.date.issued 2021
• dc.description.abstract CCCTC-binding factor (CTCF) is a conserved zinc finger transcription factor implicated in a wide range of functions, including genome organization, transcription activation, and elongation. To explore the basis for CTCF functional diversity, we coupled an auxin-induced degron system with precision nuclear run-on. Unexpectedly, oriented CTCF motifs in gene bodies are associated with transcriptional stalling in a manner independent of bound CTCF. Moreover, CTCF at different binding sites (CBSs) displays highly variable resistance to degradation. Motif sequence does not significantly predict degradation behavior, but location at chromatin boundaries and chromatin loop anchors, as well as co-occupancy with cohesin, are associated with delayed degradation. Single-molecule tracking experiments link chromatin residence time to CTCF degradation kinetics, which has ramifications regarding architectural CTCF functions. Our study highlights the heterogeneity of CBSs, uncovers properties specific to architecturally important CBSs, and provides insights into the basic processes of genome organization and transcription regulation.
• dc.description.sponsorship This work was supported by NIH grants R01 DK054937 to G.A.B., R24 DK106766 to G.A.B. and R.C.H., and R01GM121613 to R.C.H
• dc.format.mimetype application/pdf
• dc.identifier.citation Luan J, Xiang G, Gómez-García PA, Tome JM, Zhang Z, Vermunt MW et al. Distinct properties and functions of CTCF revealed by a rapidly inducible degron system. Cell Rep. 2021 Feb 23;34(8):108783. DOI:10.1016/j.celrep.2021.10878
• dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2021.108783
• dc.identifier.issn 2211-1247
• dc.identifier.uri http://hdl.handle.net/10230/52910
• dc.language.iso eng
• dc.publisher Cell Press
• dc.rights © 2021 Jing Luan et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.other Genòmica
• dc.subject.other Cromatina
• dc.title Distinct properties and functions of CTCF revealed by a rapidly inducible degron system
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion