Histopathological and ultrastructural changes after electroporation in pig liver using parallel-plate electrodes and high-performance generator

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• dc.contributor.author López-Alonso, B.
• dc.contributor.author Hernáez, Álvaro
• dc.contributor.author Sarnago, Héctor
• dc.contributor.author Naval, Alejandro
• dc.contributor.author Güemes, A.
• dc.contributor.author Junquera, C.
• dc.contributor.author Burdío, José M.
• dc.contributor.author Castiella, T.
• dc.contributor.author Monleón, E.
• dc.contributor.author Gracía-Llanes, J.
• dc.contributor.author Burdío Pinilla, Fernando
• dc.contributor.author Mejía, E.
• dc.contributor.author Lucía, Óscar
• dc.date.accessioned 2020-04-20T07:25:23Z
• dc.date.available 2020-04-20T07:25:23Z
• dc.date.issued 2019
• dc.description.abstract Irreversible electroporation (IRE) has gained attention as a new non-thermal therapy for ablation with important benefits in terms of homogeneous treatment and fast recovery. In this study, a new concept of high voltage generator is used, enabling irreversible electroporation treatment in large tissue volume using parallel plates. Unlike currently available generators, the proposed versatile structure enables delivering high-voltage high-current pulses. To obtain homogeneous results, 3-cm parallel-plates electrodes have also been designed and implemented. IRE ablation was performed on six female pigs at 2000 V/cm electric field, and the results were analysed after sacrifice three hours, three days and seven days after ablation. Histopathological and ultrastructural studies, including transmission and scanning electron microscopy, were carried out. The developed high-voltage generator has proved to be effective for homogeneous IRE treatment using parallel plates. The destruction of the membrane of the hepatocytes and the alterations of the membranes of the cellular organelles seem incompatible with cell death by apoptosis. Although endothelial cells also die with electroporation, the maintenance of vascular scaffold allows repairing processes to begin from the third day after IRE as long as the blood flow has not been interrupted. This study has opened new direction for IRE using high performance generators and highlighted the importance of taking into account ultrastructural changes after IRE by using electron microscopy analysis.
• dc.description.sponsorship This work was partly supported by the Spanish MINECO under Project TEC2016-78358-R and by the DGA-FSE. Authors would like to acknowledge the use of Servicio General de Apoyo a la Investigación-SAI, Universidad de Zaragoza.
• dc.format.mimetype application/pdf
• dc.identifier.citation López-Alonso B, Hernáez A, Sarnago H, Naval A, Güemes A, Junquera C, et al. Histopathological and ultrastructural changes after electroporation in pig liver using parallel-plate electrodes and high-performance generator. Sci Rep. 2019 Feb 25; 9(1): 2647. DOI: 10.1038/s41598-019-39433-6
• dc.identifier.doi http://dx.doi.org/10.1038/s41598-019-39433-6
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/44271
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2016-78358-R
• dc.rights © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other Electroforesi
• dc.subject.other Fetge -- Malalties -- Tractament
• dc.subject.other Histopatologia
• dc.title Histopathological and ultrastructural changes after electroporation in pig liver using parallel-plate electrodes and high-performance generator
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion