The function of GORASPs in Golgi apparatus organization in vivo

Grond, Rianne; Veenendaal, Tineke; Duran, Juan M.; Raote, Ishier; Van Es, Johan H.; Corstjens, Sebastiaan; Delfgou, Laura; El Haddouti, Benaissa; Malhotra, Vivek; Rabouille, Catherine

The function of GORASPs in Golgi apparatus organization in vivo

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dc.contributor.author Grond, Rianne
dc.contributor.author Veenendaal, Tineke
dc.contributor.author Duran, Juan M.
dc.contributor.author Raote, Ishier
dc.contributor.author Van Es, Johan H.
dc.contributor.author Corstjens, Sebastiaan
dc.contributor.author Delfgou, Laura
dc.contributor.author El Haddouti, Benaissa
dc.contributor.author Malhotra, Vivek
dc.contributor.author Rabouille, Catherine
dc.date.accessioned 2020-11-23T07:03:48Z
dc.date.issued 2020
dc.description.abstract In vitro experiments have shown that GRASP65 (GORASP1) and GRASP55 (GORASP2) proteins function in stacking Golgi cisternae. However, in vivo depletion of GORASPs in metazoans has given equivocal results. We have generated a mouse lacking both GORASPs and find that Golgi cisternae remained stacked. However, the stacks are disconnected laterally from each other, and the cisternal cross-sectional diameters are significantly reduced compared with their normal counterparts. These data support earlier findings on the role of GORASPs in linking stacks, and we suggest that unlinking of stacks likely affects dynamic control of COPI budding and vesicle fusion at the rims. The net result is that cisternal cores remain stacked, but cisternal diameter is reduced by rim consumption.
dc.description.sponsorship V. Malhotra is an Institució Catalana de Recerca i Estudis Avançats professor at the Centre for Genomic Regulation, and work in his laboratory is funded by the Ministerio de Economía y Competitividad (grants SEV-2012-0208, BFU2013-44188-P, and CSD2009-00016). I. Raote acknowledges funding from the Spanish Ministry of Science and Innovation (IJCI-2017-34751).
dc.format.mimetype application/pdf
dc.identifier.citation Grond R, Veenendaal T, Duran JM, Raote I, van Es JH, Corstjens S, Delfgou L, El Haddouti B, Malhotra V, Rabouille C. The function of GORASPs in Golgi apparatus organization in vivo. J Cell Biol. 2020; 219(9):e202004191. DOI: 10.1083/jcb.202004191
dc.identifier.doi http://dx.doi.org/10.1083/jcb.202004191
dc.identifier.issn 0021-9525
dc.identifier.uri http://hdl.handle.net/10230/45832
dc.language.iso eng
dc.publisher Rockefeller University Press
dc.relation.ispartof J Cell Biol. 2020; 219(9):e202004191
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-44188-P
dc.rights © 2020 Grond et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.keyword Organelles
dc.subject.keyword Trafficking
dc.title The function of GORASPs in Golgi apparatus organization in vivo
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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