Regulation of transcription elongation in response to osmostress

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• dc.contributor.author Silva, Andreaca
• dc.contributor.author Cavero, Santiagoca
• dc.contributor.author Begley, Victoriaca
• dc.contributor.author Solé, Carmeca
• dc.contributor.author Böttcher, Renéca
• dc.contributor.author Chávez, Sebastiánca
• dc.contributor.author Posas Garriga, Francescca
• dc.contributor.author Nadal Clanchet, Eulàlia deca
• dc.date.accessioned 2018-03-21T08:06:21Z
• dc.date.available 2018-03-21T08:06:21Z
• dc.date.issued 2017
• dc.description.abstract Cells trigger massive changes in gene expression upon environmental fluctuations. The Hog1 stress-activated protein kinase (SAPK) is an important regulator of the transcriptional activation program that maximizes cell fitness when yeast cells are exposed to osmostress. Besides being associated with transcription factors bound at target promoters to stimulate transcriptional initiation, activated Hog1 behaves as a transcriptional elongation factor that is selective for stress-responsive genes. Here, we provide insights into how this signaling kinase functions in transcription elongation. Hog1 phosphorylates the Spt4 elongation factor at Thr42 and Ser43 and such phosphorylations are essential for the overall transcriptional response upon osmostress. The phosphorylation of Spt4 by Hog1 regulates RNA polymerase II processivity at stress-responsive genes, which is critical for cell survival under high osmostress conditions. Thus, the direct regulation of Spt4 upon environmental insults serves to stimulate RNA Pol II elongation efficiency.
• dc.description.sponsorship AS was a recipient of an EMBO Long-Term Fellowship (EMBO ALTF 1394-2012). This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-64437-P and FEDER, BFU2014-52125-REDT, BFU2014-51672-REDC to FP; BFU2014-52333-P and FEDER to EdN; BFU2016-77728-C3-1-P and BFU2013-48643-C3-1-P to SC), the Junta de Andalucía (P12-BIO1938MO to SC) and the Catalan Government (2014 SGR 599 to FP and EdN). This project was also supported by Fundación Botín, by Banco Santander through its Santander Universities Global Division to FP. FP is recipient of an ICREA Acadèmia (Generalitat de Catalunya).
• dc.format.mimetype application/pdf
• dc.identifier.citation Silva A, Cavero S, Begley V, Solé C, Bottcher R, Chávez S et al. Regulation of transcription elongation in response to osmostress. PLoS Genet. 2017 Nov 20;13(11):e1007090. DOI: 10.1371/journal.pgen.1007090
• dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1007090
• dc.identifier.issn 1553-7390
• dc.identifier.uri http://hdl.handle.net/10230/34223
• dc.language.iso eng
• dc.publisher Public Library of Science (PLoS)ca
• dc.relation.ispartof PLOS Genetics. 2017 Nov 20;13(11):e1007090
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-52125-REDT
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-51672-REDC
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-52333-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-77728-C3-1-P
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2013-48643-C3-1-P
• dc.rights © 2017 Silva et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Phosphorylation
• dc.subject.keyword Osmotic shock
• dc.subject.keyword Transcriptional control
• dc.subject.keyword Gene expression regulation
• dc.title Regulation of transcription elongation in response to osmostressca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion