Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum

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  • dc.contributor.author Wang, Sihui
  • dc.contributor.author Idrissi, Fatima-Zahra
  • dc.contributor.author Hermansson, Martin
  • dc.contributor.author Grippa, Alexandra, 1980-
  • dc.contributor.author Ejsing, Christer S.
  • dc.contributor.author Carvalho, Pedro C.
  • dc.date.accessioned 2019-11-25T08:32:04Z
  • dc.date.available 2019-11-25T08:32:04Z
  • dc.date.issued 2018
  • dc.description.abstract Lipid droplets (LDs) and peroxisomes are ubiquitous organelles with central roles in eukaryotic cells. Although the mechanisms involved in biogenesis of these organelles remain elusive, both seem to require the endoplasmic reticulum (ER). Here we show that in yeast the ER budding of these structurally unrelated organelles has remarkably similar requirements and involves cooperation between Pex30 and the seipin complex. In the absence of these components, budding of both LDs and peroxisomes is inhibited, leading to the ER accumulation of their respective constituent molecules, such as triacylglycerols and peroxisomal membrane proteins, whereas COPII vesicle formation remains unaffected. This phenotype can be reversed by remodeling ER phospholipid composition highlighting a key function of these lipids in organelle biogenesis. We propose that seipin and Pex30 act in concert to organize membrane domains permissive for organelle budding, and that may have a lipid composition distinct from the bulk ER.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Wang S, Idrissi FZ, Hermansson M, Grippa A, Ejsing CS, Carvalho P. Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum. Nat Commun. 2018;9(1):2939. DOI: 10.1038/s41467-018-05278-2
  • dc.identifier.doi http://dx.doi.org/10.1038/s41467-018-05278-2
  • dc.identifier.issn 2041-1723
  • dc.identifier.uri http://hdl.handle.net/10230/42951
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Nature Communications. 2018;9(1):2939
  • dc.rights © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Endoplasmic reticulum
  • dc.subject.keyword Peroxisomes
  • dc.title Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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