Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB

Coelho, Miguel B.; Attig, Jan; Bellora Pereyra, Nicolás; König, Julian; Hallegger, Martina; Kayikci, Melis; Eyras Jiménez, Eduardo; Ule, Jernej; Smith, Christopher, W. J.

Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB

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dc.contributor.author Coelho, Miguel B.ca
dc.contributor.author Attig, Janca
dc.contributor.author Bellora Pereyra, Nicolásca
dc.contributor.author König, Julianca
dc.contributor.author Hallegger, Martinaca
dc.contributor.author Kayikci, Melisca
dc.contributor.author Eyras Jiménez, Eduardoca
dc.contributor.author Ule, Jernejca
dc.contributor.author Smith, Christopher, W. J.ca
dc.date.accessioned 2016-02-10T16:14:11Z
dc.date.available 2016-02-10T16:14:11Z
dc.date.issued 2015
dc.description.abstract Matrin3 is an RNA- and DNA-binding nuclear matrix protein found to be associated with neural and muscular degenerative diseases. A number of possible functions of Matrin3 have been suggested, but no widespread role in RNA metabolism has yet been clearly demonstrated. We identified Matrin3 by its interaction with the second RRM domain of the splicing regulator PTB. Using a combination of RNAi knockdown, transcriptome profiling and iCLIP, we find that Matrin3 is a regulator of hundreds of alternative splicing events, principally acting as a splicing repressor with only a small proportion of targeted events being co-regulated by PTB. In contrast to other splicing regulators, Matrin3 binds to an extended region within repressed exons and flanking introns with no sharply defined peaks. The identification of this clear molecular function of Matrin3 should help to clarify the molecular pathology of ALS and other diseases caused by mutations of Matrin3.ca
dc.description.sponsorship This work was supported by Wellcome Trust programme grants to CWJS (077877 and 092900) and by grants to EE and NB BIO2011-23920 and RNAREG (CSD2009-00080) from the Spanish Government and by the Sandra Ibarra Foundation for Cancer (FSI 2013). JA was supported by a Boehringer Ingelheim Fonds studentship
dc.format.mimetype application/pdfca
dc.identifier.citation Coelho MB, Attig J, Bellora N, König J, Hallegger M, Kayikci M et al. Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB. The EMBO Journal. 2015;34(5):653-68. DOI: 10.15252/embj.201489852ca
dc.identifier.doi http://dx.doi.org/10.15252/embj.201489852
dc.identifier.issn 0261-4189
dc.identifier.uri http://hdl.handle.net/10230/25774
dc.language.iso engca
dc.publisher EMBO Pressca
dc.relation.ispartof The EMBO Journal. 2015;34(5):653-68
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2011-23920
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CSD2009-00080
dc.rights © 2015 The authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly citedca
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ca
dc.subject.keyword Alternative splicing
dc.subject.keyword Matrin 3
dc.subject.keyword PTB
dc.subject.other RNAca
dc.subject.other ADNca
dc.title Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTBca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

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