Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation

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  • dc.contributor.author Escobar-Niño, Almudena
  • dc.contributor.author Liñeiro, Eva
  • dc.contributor.author Amil, Francisco
  • dc.contributor.author Carrasco, Raphaël, 1950-
  • dc.contributor.author Chiva, Cristina
  • dc.contributor.author Fuentes, Carlos
  • dc.contributor.author Blanco-Ulate, Barbara
  • dc.contributor.author Cantoral Fernández, Jesús M.
  • dc.contributor.author Sabidó Aguadé, Eduard, 1981-
  • dc.contributor.author Fernández-Acero, Francisco Javier
  • dc.date.accessioned 2019-10-01T08:06:16Z
  • dc.date.available 2019-10-01T08:06:16Z
  • dc.date.issued 2019
  • dc.description.abstract Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins predicted in the genome database of this fungus. To increase coverage, we analysed by LC-MS/MS the first-reported overlapped proteome in phytopathogenic fungi, the "phosphomembranome" of B. cinerea, combining the two most important signal transduction subproteomes. Of the 1112 membrane-associated phosphoproteins identified, 64 and 243 were classified as exclusively identified or overexpressed under glucose and deproteinized tomato cell wall conditions, respectively. Seven proteins were found under both conditions, but these presented a specific phosphorylation pattern, so they were considered as exclusively identified or overexpressed proteins. From bioinformatics analysis, those differences in the membrane-associated phosphoproteins composition were associated with various processes, including pyruvate metabolism, unfolded protein response, oxidative stress response, autophagy and cell death. Our results suggest these proteins play a significant role in the B. cinerea pathogenic cycle.
  • dc.description.sponsorship This research has been financed by the Spanish Government under project AGL2015-65684-C2-2-R supported by the Spanish Ministry of Economy and Competitiveness. Eva Liñeiro was supported by an FPI grant from the University of Cádiz (2010-152). The CRG/UPF Proteomics Unit is a member of the ProteoRed PRB3 consortium which is supported by grant PT17/0019 of the PE I+D+i 2013–2016 from the Instituto de Salud Carlos III (ISCIII) and ERDF. We acknowledge support from the Spanish Ministry of Economy and Competitiveness, “Centro de Excelencia Severo Ochoa 2013–2017”, SEV-2012-0208, and “Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya” (2017SGR595).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Escobar-Niño A, Liñeiro E, Amil F, Carrasco R, Chiva C, Fuentes C et al. Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation. Scientific Reports. 2019;9(1):9860. DOI: 10.1038/s41598-019-46270-0
  • dc.identifier.doi http://dx.doi.org/10.1038/s41598-019-46270-0
  • dc.identifier.issn 2045-2322
  • dc.identifier.uri http://hdl.handle.net/10230/42364
  • dc.language.iso eng
  • dc.publisher Nature Research
  • dc.relation.ispartof Scientific Reports. 2019;9(1):9860
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2015-65684-C2-2-R
  • dc.rights © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Fungal pathogenesis
  • dc.subject.keyword Protein–protein interaction networks
  • dc.title Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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