p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes

Perearnau, Anna; Orlando, Serena; Islam, Abul, 1978-; Gallastegui Calvache, Edurne; Martínez, Jonatan; Jordan Vallès, Albert; Bigas Salvans, Anna; Aligué, Rosa; Pujol, Maria Jesús; Bachs Valldeneu, Oriol

p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes

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dc.contributor.author Perearnau, Annaca
dc.contributor.author Orlando, Serenaca
dc.contributor.author Islam, Abul, 1978-ca
dc.contributor.author Gallastegui Calvache, Edurneca
dc.contributor.author Martínez, Jonatanca
dc.contributor.author Jordan Vallès, Albertca
dc.contributor.author Bigas Salvans, Annaca
dc.contributor.author Aligué, Rosaca
dc.contributor.author Pujol, Maria Jesúsca
dc.contributor.author Bachs Valldeneu, Oriolca
dc.date.accessioned 2018-02-06T09:00:07Z
dc.date.available 2018-02-06T09:00:07Z
dc.date.issued 2017
dc.description.abstract The cyclin-dependent kinase inhibitor p27Kip1 (p27) also behaves as a transcriptional repressor. Data showing that the p300/CBP-associated factor (PCAF) acetylates p27 inducing its degradation suggested that PCAF and p27 could collaborate in the regulation of transcription. However, this possibility remained to be explored. We analyzed here the transcriptional programs regulated by PCAF and p27 in the colon cancer cell line HCT116 by chromatin immunoprecipitation sequencing (ChIP-seq). We identified 269 protein-encoding genes that contain both p27 and PCAF binding sites being the majority of these sites different for PCAF and p27. PCAF or p27 knock down revealed that both regulate the expression of these genes, PCAF as an activator and p27 as a repressor. The double knock down of PCAF and p27 strongly reduced their expression indicating that the activating role of PCAF overrides the repressive effect of p27. We also observed that the transcription factor Pax5 interacts with both p27 and PCAF and that the knock down of Pax5 induces the expression of p27/PCAF target genes indicating that it also participates in the transcriptional regulation mediated by p27/PCAF. In summary, we report here a previously unknown mechanism of transcriptional regulation mediated by p27, Pax5 and PCAF.
dc.format.mimetype application/pdf
dc.identifier.citation Perearnau A1, Orlando S1, Islam ABMMK2, Gallastegui E1, Martínez J1, Jordan A. et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res. 2017 May 19;45(9):5086-5099. DOI: 10.1093/nar/gkx075
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkx075
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10230/33806
dc.language.iso eng
dc.publisher Oxford University Pressca
dc.relation.ispartof Nucleic Acids Research. 2017 May 19;45(9):5086-99
dc.rights Copyright © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.subject.other Regulació genètica
dc.subject.other Cèl·lules
dc.subject.other Cromatina
dc.title p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genesca
dc.type info:eu-repo/semantics/article
dc.type.version info:eu-repo/semantics/publishedVersion

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