Genetic and protein biomarkers in blood for the improved detection of GH abuse
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- dc.contributor.author Ferro, P.ca
- dc.contributor.author Ventura Alemany, Rosaca
- dc.contributor.author Pérez Mañá, Claraca
- dc.contributor.author Farré Albaladejo, Magíca
- dc.contributor.author Segura, Joanca
- dc.date.accessioned 2016-10-06T09:33:00Z
- dc.date.issued 2016
- dc.description.abstract Human Growth Hormone (hGH, somatotropin) is one of the relevant forbidden substances to be detected in sport drug testing. Since the appearance of recombinant hGH (rhGH) in the 80's, its expansion and availability through the black market have increased, so the detection of its abuse continues to be a challenge at present. New techniques or biomarkers that are robust, reliable, sensitive and allowing a large detection time window are welcome. rhGH produces an increase of insulin-like growth factor 1 (IGF-1). FN1 (fibronectin 1) and RAB31 (member of RAS oncogene family) genes have been suggested as two potential biomarkers for IGF-1 abuse. Following this line, in the present study some genetic and proteomic approaches have been performed with fourteen healthy male subjects treated with rhGH (which produces increase of IGF-1 concentrations) to study FN1 gene, FN1 protein, RAB31 gene and RAB31 protein as potential biomarkers for rhGH abuse. The results showed that both, RAB31 and FN1 genes and FN1 protein could be potential biomarkers for rhGH administration. Preliminary assessments of gender, age, acute sport activities and GHRP-2 (pralmorelin, a rhGH releasing peptide) influence suggest they are not relevant confounding factors. Thus, the selected markers present high sensitivity and a larger detection window for rhGH detection than IGF-1 itself.ca
- dc.description.sponsorship This work was carried out with the partial financial support of the International Olympic Committee (2014 IOC Anti-Doping Research Fund) and the Spanish Ministerio de Economía y Competividad (MINECO) (DEP2012-32048). Clara Pérez-Manᘠholds a Juan Rodes fellowship (ISC-III, JR15/00005).
- dc.format.mimetype application/pdfca
- dc.identifier.citation Ferro P, Ventura R, Pérez-Mañá C, Farré M, Segura J. Genetic and protein biomarkers in blood for the improved detection of GH abuse. J Pharm Biomed Anal. 2016 Sep 5;128:111-8. doi: 10.1016/j.jpba.2016.05.022ca
- dc.identifier.doi http://dx.doi.org/10.1016/j.jpba.2016.05.022
- dc.identifier.issn 0731-7085
- dc.identifier.uri http://hdl.handle.net/10230/27387
- dc.language.iso engca
- dc.publisher Elsevierca
- dc.relation.ispartof Journal of Pharmaceutical and Biomedical Analysis. 2016 Sep 5;128:111-8
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/DEP2012-32048
- dc.rights © Elsevier http://dx.doi.org/10.1016/j.jpba.2016.05.022ca
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.other Dopatge en els esportsca
- dc.subject.other Marcadors bioquímicsca
- dc.title Genetic and protein biomarkers in blood for the improved detection of GH abuseca
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/acceptedVersionca