Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria

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• dc.contributor.author Vidovic, Anita
• dc.contributor.author Supek, Fran
• dc.contributor.author Nikolic, Andrea
• dc.contributor.author Krisko, Anita
• dc.date.accessioned 2025-01-21T07:38:34Z
• dc.date.available 2025-01-21T07:38:34Z
• dc.date.issued 2014
• dc.description.abstract Protein oxidation is known to compromise vital cellular functions. Therefore, invading pathogenic bacteria must resist damage inflicted by host defenses via reactive oxygen species. Using comparative genomics and experimental approaches, we provide multiple lines of evidence that proteins from pathogenic bacteria have acquired resistance to oxidative stress by an increased conformational stability. Representative pathogens exhibited higher survival upon HSP90 inhibition and a less-oxidation-prone proteome. A proteome signature of the 46 pathogenic bacteria encompasses 14 physicochemical features related to increasing protein conformational stability. By purifying ten representative proteins, we demonstrate in vitro that proteins with a pathogen-like signature are more resistant to oxidative stress as a consequence of their increased conformational stability. A compositional signature of the pathogens’ proteomes allowed the design of protein fragments more resilient to both unfolding and carbonylation, validating the relationship between conformational stability and oxidability with implications for synthetic biology and antimicrobial strategies.en
• dc.description.sponsorship The authors thank Miroslav Radman for stimulating discussions and Tea Copic for technical support. This work was supported by the Mediterranean Institute for Life Sciences. A.V. and A.N. are supported by the City of Split Fellowship for Excellence. The work of F.S. was supported in part by Marie Curie Actions; by grants 00980501 and 098-0000000-3168 of the Ministry of Science, Education, and Sport of Croatia; and by EU grant ICT-2013-612944 (MAESTRA). A.K. would like to thank Maria Marta Radman for her help with the graphics.en
• dc.format.mimetype application/pdf
• dc.identifier.citation Vidovic A, Supek F, Nikolic A, Krisko A. Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria. Cell Reports. 2014 Jun;7(5):1393-400. DOI: 10.1016/j.celrep.2014.04.057
• dc.identifier.issn 2211-1247
• dc.identifier.uri http://hdl.handle.net/10230/69213
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Cell Reports. 2014 Jun;7(5):1393-400
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/612944
• dc.rights © 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
• dc.subject.other Proteòmicaca
• dc.subject.other Bacteris patògensca
• dc.subject.other Oxidacióca
• dc.title Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteriaen
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion