Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells

Muñoz-Gil, Gorka; Romero-Aristizabal, Catalina; Mateos, Nicolas; Campelo, Felix; De Llobet, Lara Isabel; Beato, Miguel; Lewenstein, Maciej; Garcia-Parajo, Maria F.; Torreno-Pina, Juan A.

doi:http://dx.doi.org/10.1073/pnas.2200667119

Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells

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Muñoz-Gil G, Romero-Aristizabal C, Mateos N, Campelo F, de Llobet Cucalon LI, Beato M, Lewenstein M, Garcia-Parajo MF, Torreno-Pina JA. Stochastic particle unbinding modulates growth dynamics and size of transcription factor condensates in living cells. Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2200667119. DOI: 10.1073/pnas.2200667119

Abstract

Liquid-liquid phase separation (LLPS) is emerging as a key physical principle for biological organization inside living cells, forming condensates that play important regulatory roles. Inside living nuclei, transcription factor (TF) condensates regulate transcriptional initiation and amplify the transcriptional output of expressed genes. However, the biophysical parameters controlling TF condensation are still poorly understood. Here we applied a battery of single-molecule imaging, theory, and simulations to investigate the physical properties of TF condensates of the progesterone receptor (PR) in living cells. Analysis of individual PR trajectories at different ligand concentrations showed marked signatures of a ligand-tunable LLPS process. Using a machine learning architecture, we found that receptor diffusion within condensates follows fractional Brownian motion resulting from viscoelastic interactions with chromatin. Interestingly, condensate growth dynamics at shorter times is dominated by Brownian motion coalescence (BMC), followed by a growth plateau at longer timescales that result in nanoscale condensate sizes. To rationalize these observations, we extended on the BMC model by including the stochastic unbinding of particles within condensates. Our model reproduced the BMC behavior together with finite condensate sizes at the steady state, fully recapitulating our experimental data. Overall, our results are consistent with condensate growth dynamics being regulated by the escaping probability of PR molecules from condensates. The interplay between condensation assembly and molecular escaping maintains an optimum physical condensate size. Such phenomena must have implications for the biophysical regulation of other nuclear condensates and could also operate in multiple biological scenarios.